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  • © 2001

Angiogenesis Protocols

Part of the book series: Methods in Molecular Medicine (MIMM, volume 46)

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  • ISBN: 978-1-59259-143-5
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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Special Review Article

    1. Front Matter

      Pages 1-1
    2. Therapeutic Inhibition of Angiogenesis

      • Hua-Tang Zhang, Roy Bicknell
      Pages 3-26
  3. Angiogenesis Protocols in Vivo

    1. Front Matter

      Pages 27-27
    2. In Vivo Matrigel Migration and Angiogenesis Assays

      • Katherine M. Malinda
      Pages 47-52
    3. Alginate Microbead Release Assay of Angiogenesis

      • Clifford Y. Ko, Vivek Dixit, William W. Shaw, Gary Gitnick
      Pages 53-57
    4. Disc Angiogenesis Assay

      • Anthony C. Allison, Luis-F. Fajardo
      Pages 59-75
    5. Sponge Implant Model of Angiogenesis

      • Silvia P. Andrade
      Pages 77-86
    6. Hollow Fiber Assay for Tumor Angiogenesis

      • Roger M. Phillips, Michael C. Bibby
      Pages 87-93
    7. Dorsal Skinfold Chamber Preparation in Mice

      • Axel Sckell, Michael Leunig
      Pages 95-105
    8. Angiogenesis Assays Using Chick Chorioallantoic Membrane

      • David C. West, W. Douglas Thompson, Paula G. Sells, Mike F. Burbridge
      Pages 107-129
    9. Corneal Assay for Angiogenesis

      • Marina Ziche
      Pages 131-142
  4. Angiogenesis Protocols in Vitro

    1. Front Matter

      Pages 143-143
    2. Collagen Gel Assay for Angiogenesis

      • Ana M. Schor, Ian Ellis, Seth L. Schor
      Pages 145-162
    3. Chemotaxis and Chemokinesis in 3D Macromolecular Matrices

      • Ana M. Schor, Ian Ellis, Seth L. Schor
      Pages 163-183
    4. Rat Aortic Ring

      • Mike F. Burbridge, David C. West
      Pages 185-204
    5. In Vitro Matrigel Angiogenesis Assays

      • M. Lourdes Ponce
      Pages 205-209
  5. Associated Techniques

    1. Front Matter

      Pages 211-211

About this book

Although angiogenesis-the formation of new blood vessels from pre-existing ones-has emerged as an exciting new target for the treatment of many varied disorders, fundamental aspects of this important biological process remain poorly understood. To address the needs of those working in such a dramatically advancing area, Cliff Murray and a panel of experienced investigators have brought together in Angiogenesis Protocols a comprehensive collection of key techniques for assessing the formation of new blood vessels in both whole tissues and cell culture. These readily reproducible methods range from low-cost in vitro assays to cutting-edge in vivo models, such as the dorsal window chamber, which demands high levels of surgical skill as well as relatively expensive, custom-made equipment. The authors have also included details of the associated techniques for the isolation, identification, and culture of microvessel endothelial cells from human adipose tissues and for the transfection and transduction of primary human endothelial cells.
Timely and highly practical, Angiogenesis Protocols is a gold-standard resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources.

Reviews

"There is an excellent overview of existing inhibitors of angiogenesis, including both natural and man-made and a review of the biological features that define efficacy and specificity of anti-angiogenic therapies. Most existing in vivo angiogenesis assays are combined in one edition. New microvessel markers are listed to allow more accurate microvessel counts. The details on assays that were proprietary only to a few labs nationwide, such as dorsal skinfold, chamber, and corneal neovascularization assays, are now available to the general science community. Given the well known difficulties of transfection of the primary endothelial cultures, the chapter summarizing information available on transfection and transduction of these cells is particularly useful. . .This is the first book offering such a variety of angiogenesis assays to the general reader where the scientific basis is given in accessible language and technical procedures are readily available. Its publication will undoubtedly facilitate new experiments and further therapeutic angiogenesis."-Doody's Health Sciences Book Review Journal

"The book provides an excellent review concerning anti-angiogenesis. Inhibiting angiogenesis is an important topic in cancer control. . .the book would be a convenient reference for any investigator or laboratory interested in angiogenesis. Angiogenesis Protocols is recommended to any research orientated health science library."-E-Streams Electronic Book Review Journal

"Timely and highly practical, Angiogenesis Protocols is an important resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources." - Anticancer Research

Editors and Affiliations

  • University of Nottingham and City Hospital, Nottingham, UK

    J. Clifford Murray

Bibliographic Information

  • Book Title: Angiogenesis Protocols

  • Editors: J. Clifford Murray

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591434

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • eBook ISBN: 978-1-59259-143-5

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: X, 267

  • Number of Illustrations: 119 b/w illustrations

  • Topics: Cell Biology

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-143-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout