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SV40 Protocols

  • Leda Raptis

Part of the Methods in Molecular Biology™ book series (MIMB, volume 165)

Table of contents

  1. Front Matter
    Pages i-xi
  2. James D. Tremblay, Kris F. Sachsenmeier, James M. Pipas
    Pages 1-7
  3. Christine K. Liu, Walter J. Atwood
    Pages 9-17
  4. Wendy A. Knowles
    Pages 19-31
  5. Kodangattil R. Sreekumar, Brett A. Barbaro, Andrea E. Prack, Peter A. Bullock
    Pages 49-67
  6. Richard J. Kraus, Janet E. Mertz
    Pages 69-85
  7. Richard J. Kraus, Janet E. Mertz
    Pages 87-101
  8. David S. Strayer, Maria Lamothe, Danlan Wei, Joseph Milano, Rumi Kondo
    Pages 103-117
  9. Van Cherington, Cynthia Higgins
    Pages 129-150
  10. Leda Raptis, Adina Vultur
    Pages 151-164
  11. Mary Judith Tevethia, Harvey L. Ozer
    Pages 185-199
  12. Ximena Montano
    Pages 201-211
  13. Heather L. Brownell, Leda Raptis
    Pages 219-228
  14. Ximena Montano, Kathy Rundell
    Pages 229-242
  15. Todd D. Schell, Satvir S. Tevethia
    Pages 243-256
  16. John A. Lednicky, Robert L. Garcea
    Pages 257-267
  17. Ioanna G. Maroulakou, Robin C. Muise-Helmericks
    Pages 269-286
  18. Back Matter
    Pages 287-310

About this book

Introduction

Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources.
Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.

Editors and affiliations

  • Leda Raptis
    • 1
  1. 1.Queen’s UniversityKingstonCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592591175
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-653-6
  • Online ISBN 978-1-59259-117-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site