SV40 Protocols

1. Front Matter

   Pages i-xi

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2. Propagation of Wild-Type and Mutant SV40

   • James D. Tremblay, Kris F. Sachsenmeier, James M. Pipas

   Pages 1-7

3. Propagation and Assay of the JC Virus

   • Christine K. Liu, Walter J. Atwood

   Pages 9-17

4. Propagation and Assay of BK Virus

   • Wendy A. Knowles

   Pages 19-31

5. Molecular Methods for Identification and Genotyping of BK Virus

   • Li Jin

   Pages 33-48

6. Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replication

   • Kodangattil R. Sreekumar, Brett A. Barbaro, Andrea E. Prack, Peter A. Bullock

   Pages 49-67

7. Cell-Free Transcription of SV40

   • Richard J. Kraus, Janet E. Mertz

   Pages 69-85

8. Quantitation and Structural Analysis of SV40 RNAs

   • Richard J. Kraus, Janet E. Mertz

   Pages 87-101

9. Generation of Recombinant SV40 Vectors for Gene Transfer

   • David S. Strayer, Maria Lamothe, Danlan Wei, Joseph Milano, Rumi Kondo

   Pages 103-117

10. Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions

    • Ziv Sandalon, Ariella Oppenheim

    Pages 119-128

11. Using Retroviral Vectors to Express SV40 Tumor Antigens

    • Van Cherington, Cynthia Higgins

    Pages 129-150

12. Neoplastic Transformation Assays

    • Leda Raptis, Adina Vultur

    Pages 151-164

13. Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen

    • Cynthia Higgins, Van Cherington

    Pages 165-183

14. SV40-Mediated Immortalization

    • Mary Judith Tevethia, Harvey L. Ozer

    Pages 185-199

15. Biology of p53 and SV40 Large T Association

    • Ximena Montano

    Pages 201-211

16. Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins

    • Juan Zalvide, James A. DeCaprio, Hilde Stubdal

    Pages 213-218

17. In situ Electroporation for the Measurement of c-Ras Activation by SVLT

    • Heather L. Brownell, Leda Raptis

    Pages 219-228

18. Role of SV40 Small t in Cell Lysis, Transformation, and Signaling

    • Ximena Montano, Kathy Rundell

    Pages 229-242

19. Cytotoxic T Lymphocytes in SV40 Infections

    • Todd D. Schell, Satvir S. Tevethia

    Pages 243-256

20. Detection of SV40 DNA Sequences in Human Tissue

    • John A. Lednicky, Robert L. Garcea

    Pages 257-267

Simian virus 40 gained notoriety in the 1960s because it was found to be a contaminant of polio and adenovirus vaccines that had been administered to millions of healthy individuals worldwide. The public health implications of this revelation provided the initial impetus for an in-depth study of SV40 biology. Later work showed that SV40 DNA sequences as well as infectious virus are in fact found in human tumors and may have contributed to oncog- esis. It also turned out that SV40 uses mostly cellular machinery to carry out many steps in viral infection, which makes it a powerful probe for examining many fundamental questions in eukaryotic molecular biology. SV40 Pro- cols consolidates a number of well-tested step-by-step techniques in one v- ume; experts with hands-on experience in particular methods give detailed accounts of their optimized experimental protocols, so that the beginner, as well as more experienced researchers, may readily overcome problems of ambiguity often present in the literature. As with other DNA tumor viruses, the response of cultured cells to SV40 infection depends upon the species being infected. Monkey cells s- port virus production, which leads to their death, whereas rodent cells p- duce only the early proteins and acquire a transformed phenotype. Thus, SV40 Protocols is organized in two sections. The first relates to assays of the lytic cycle of the virus, and the second deals with transformation.

• Queen’s University, Kingston, Canada

  Leda Raptis

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