Capillary Electrophoresis of Nucleic Acids

Volume II: Practical Applications of Capillary Electrophoresis

  • Keith R. Mitchelson
  • Jing Cheng

Part of the Methods in Molecular Biology™ book series (MIMB, volume 163)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Practical Applications for Rapid DNA Fragment Sizing and Analysis

    1. Front Matter
      Pages 3-45
    2. H. Michael Wenz, David Dailey, Martin D. Johnson
      Pages 3-17
    3. Karel Klepárník, Odilo M. Müeller, František Foret
      Pages 19-39
    4. Haleem J. Issaq, King C. Chan
      Pages 41-45
  3. Practical Applications for Mutation Detection

    1. Front Matter
      Pages 47-147
    2. Konstantin Khrapko, Hilary A. Coller, Xiao-Cheng Li-Sucholeiki, Paulo C. André, William G. Thilly
      Pages 57-72
    3. Cecilia Gelfi, Laura Cremoresi, Maurizio Ferrari, Pier Giorgio Righetti
      Pages 73-88
    4. Paola Carrera, Pier Giorgio Righetti, Cecilia Gelfi, Maurizio Ferrari
      Pages 95-108
    5. Kenshi Hayashi, H. Michael Wenz, Masakazu Inazuka, Tomoko Tahira, Tomonari Sasaki, Donald H. Atha
      Pages 109-126
    6. Takao Kasuga, Jing Cheng, Keith R. Mitchelson
      Pages 135-147
  4. Practical Applications for Genetic Analysis

    1. Front Matter
      Pages 149-239
    2. Elaine S. Mansfield, Robert B. Wilson, Paolo Fortina
      Pages 151-161
    3. Dieter Schmalzing, Lance Koutny, Aram Adourian, Dan Chisholm, Paul Matsudaira, Daniel Ehrlich
      Pages 163-173
    4. Andrea S. Kim, Xiao-Cheng Li-Sucholeiki, William G. Thilly
      Pages 175-189
    5. Jerome P. Ferrance, Braden Giordano, James P. Landers
      Pages 191-204

About this book

Introduction

The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has produced a host of DNA analytical procedures. In the
Unprecedentedly wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This second volume of the set, Practical Applications of Capillary Electrophoresis, covers techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions. Several chapters also discuss CE analysis at elevated temperatures and the use of micro-CE and array-CE devices. The companion volume, Introduction to the Capillary Electrophoresis of Nucleic Acids, offers readily reproducible methods for the analysis of small oligonucleotides and modified nucleotides, and time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.

Keywords

DNA Nucleotide PCR RNA fluorescence gene expression temperature tissue

Editors and affiliations

  • Keith R. Mitchelson
    • 1
    • 2
  • Jing Cheng
    • 3
    • 4
  1. 1.Australian Genome Research FacilityUniversity of QueenslandBrisbane
  2. 2.Walter & Eliza Hall InstituteParkvilleAustralia
  3. 3.Biochip Research and Development Center State Key Laboratory for Biomembrane and Membrane BiotechnologyTsinghua UniversityBeijingChina
  4. 4.Aviva Biosciences CorporationSan Diego

Bibliographic information

  • DOI https://doi.org/10.1385/1592591167
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-765-6
  • Online ISBN 978-1-59259-116-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book