DNA Sequencing Protocols

  • Colin A. Graham
  • Alison J. M. Hill

Part of the Methods in Molecular Biology™ book series (MIMB, volume 167)

Table of contents

  1. Front Matter
    Pages i-x
  2. Colin A. Graham, Alison J. M. Hill
    Pages 1-12
  3. Qingzhong Yu
    Pages 33-38
  4. Ulrike Gerischer, Peter Dürre
    Pages 39-51
  5. Harry Cuppens, Jean-Jacques Cassiman
    Pages 63-88
  6. Alan T. Bankier
    Pages 89-100
  7. Robert W. Blakesley
    Pages 101-112
  8. Jonathan R. E. MacBeath, Suzanna S. Harvey, Nicola J. Oldroyd
    Pages 119-152
  9. David Watts, Jonathan R. E. MacBeath
    Pages 153-170
  10. Shirley McQuaid
    Pages 171-192
  11. Colin A. Graham, Alison J. M. Hill
    Pages 193-213
  12. Anne E. Hughes
    Pages 215-223
  13. Norman J. Dovichi, JianZhong Zhang
    Pages 225-239
  14. Back Matter
    Pages 241-244

About this book

Introduction

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project.
State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.

Editors and affiliations

  • Colin A. Graham
    • 1
  • Alison J. M. Hill
    • 2
  1. 1.Northern Ireland Regional Genetics CentreBelfast City Hospital TrustNorthern Ireland, UK
  2. 2.Department of Anatomy and PhysiologyUniversity of DundeeScotland, UK

Bibliographic information

  • DOI https://doi.org/10.1385/1592591132
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-716-8
  • Online ISBN 978-1-59259-113-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book