Angiotensin Protocols

  • Donna H. Wang

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 51)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction to The Renin-Angiotensin System

    1. Front Matter
      Pages 1-1
    2. Cheryl L. Laffer, Fernando Elijovich
      Pages 23-37
  3. Transgenic and Gene Targeting Methods

    1. Front Matter
      Pages 39-39
    2. Junji Takaya, Taiji Matsusaka, Iekuni Ichikawa
      Pages 41-52
    3. Michael J. Katovich, Hong-Wei Wang, Craig H. Gelband, Mohan K. Raizada
      Pages 67-81
    4. Dagmara Mohuczy, M. Ian Phillips
      Pages 83-104
  4. Analysis of Transcriptional and Translational Mechanisms

    1. Front Matter
      Pages 105-105
    2. Christopher T. Sherman, Allan R. Brasier
      Pages 107-125
    3. Allan R. Brasier, Christopher T. Sherman, Mohammad Jamaluddin
      Pages 127-150
    4. Hong Ji, Kamakshi Krishnamurthi, Zheng Wu, Kathryn Sandberg
      Pages 171-192
  5. Assays For Determination of Mrna Levels

    1. Front Matter
      Pages 193-193
    2. Patricia E. Gallagher, Debra I. Diz
      Pages 205-213
    3. Susan Riggs Runge, Zhaoyong Hu, Marschall S. Runge
      Pages 223-233
  6. Assays For Determination of Protein Contents and Enzyme Activity

    1. Front Matter
      Pages 235-235

About this book


The renin-angiotensin system has been in the spotlight for more than 100 years because of its vital role in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling. Recent advances in molecular genetic techniques have established that the renin-angiotensin system may well hold the key to the pathogenesis of hypertension, congestive heart failure, chronic renal failure, and stroke. In Angiotensin Protocols, Donna Wang and a panel of hands-on experts describe in step-by-step detail all the major molecular, biochemical, and functional techniques now used in probing this powerful system. These range from the use of embryonic gene targeting to create mutant strains, to the functional investigation of components of the renin-angiotensin system. Following the successful pattern of the Methods in Molecular Biology™ series, each method is crafted for experimental success with detailed instructions, perceptive notes highlighting the most critical steps, and helpful tips on pitfalls to avoid.
Comprehensive and highly practical, Angiotensin Protocols translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in health and disease today.

Editors and affiliations

  • Donna H. Wang
    • 1
  1. 1.Department of MedicineMichigan State UniversityEast Lansing

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-763-2
  • Online ISBN 978-1-59259-087-2
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book