Colorectal Cancer

Methods and Protocols

  • Steven M.

Part of the Methods in Molecular Medicine book series (MIMM, volume 50)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Christopher A. Moskaluk
    Pages 1-13
  3. Jeffrey C. Harper, Reid B. Adams, Steven M. Powell
    Pages 21-24
  4. Wa′el El-Rifai, Sakari Knuutila
    Pages 25-33
  5. Svetlana D. Pack, Zhengping Zhuang
    Pages 35-50
  6. Darryl Shibata
    Pages 59-67
  7. Yann R. Parc, Kevin C. Halling
    Pages 69-79
  8. Julie M. Cunningham, David J. Tester, Stephen N. Thibodeau
    Pages 87-98
  9. William M. Grady, Lois L. Myeroff, Hongmei He, Sanford Markowitz
    Pages 99-111
  10. Lauri A. Aaltonen, Stina Roth
    Pages 167-174
  11. Lauri A. Aaltonen, Egle Avizienyte
    Pages 175-183
  12. Lauri A. Aaltonen, Stina Roth, Charis Eng
    Pages 185-193
  13. Wa’el El-Rifai, Sakari Knuutila
    Pages 195-203

About this book


Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid.
Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.

Editors and affiliations

  • Steven M.
    • 1
  1. 1.University of Virginia Health SystemCharlottesville

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-767-0
  • Online ISBN 978-1-59259-084-1
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book