Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2000

Aging Methods and Protocols

Editors:

  1. Yvonne A. Barnett

    1. School of Biomedical Sciences, University of Ulster, Northern Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Christopher R. Barnett

    1. School of Biomedical Sciences, University of Ulster, Northern Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 38)

Sections

Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
    PDF

  2. The Study of Cell Senescence and Cell Death

    1. Front Matter

      Pages 21-21
      PDF

    2. Understanding Aging

      • Bernard L. Strehler
      Pages 1-19

    3. Use of the Fibroblast Model in the Study of Cellular Senescence

      • Vincent J. Cristofalo, Craig Volker, Robert G. Allen
      Pages 23-52

    4. Human T-Cell Clones

      • Graham Pawelec
      Pages 53-61

    5. Telomeres and Replicative Senescence

      • Hector F. Valenzuela, Rita B. Effros
      Pages 63-70

    6. Detection of Molecular Events During Apoptosis by Flow Cytometry

      • Ruaidhri J. Carmody, Ana P. Costa-Pereira, Sharon L. McKenna, Tom G. Cotter
      Pages 71-83

    7. Raf-1 Protein Kinase Activity in T Cells from Aged Mice

      • Christopher J. Kirk, Richard A. Miller
      Pages 85-96

    8. Identification of Differentially Expressed Genes in Young and Senescent T Cells

      • Andrea Engel, Mahdi Adibzadeh, Graham Pawelec
      Pages 97-115

  3. Age-Related Changes to Xenobiotic Metabolizing Enzyme Systems

    1. Front Matter

      Pages 117-117
      PDF

    2. Xenobiotic-Metabolizing Enzyme Systems and Aging

      • Christopher R. Barnett, Costas Ioannides
      Pages 119-130

  4. Age-Related Changes to Defense Systems Against Biomolecule Damage

    1. Front Matter

      Pages 131-131
      PDF

    2. Assessing Age-Related Changes in Antioxidant Status

      • Iris F. F. Benzie, John J. Strain
      Pages 133-142

    3. Measurement of DNA Damage and Repair Capacity as a Function of Age Using the Comet Assay

      • Peter H. Clingen, Jillian E. Lowe, Michael H. L. Green
      Pages 143-157

  5. Age-Related Changes to Cellular Biomolecules

    1. Front Matter

      Pages 169-169
      PDF

    2. Measurement of 8-Oxo-deoxyguanosine in Lymphocytes, Cultured Cells, and Tissue Samples by HPLC with Electrochemical Detection

      • Sharon G. Wood, Catherine M. Gedik, Nicholas J. Vaughan, Andrew R. Collins
      Pages 171-178

    3. Mutation and the Aging Process

      • Yvonne A. Barnett, Christopher R. Barnett
      Pages 179-187

    4. Somatic Mutations and Aging

      • Sai-Mei Hou
      Pages 189-197
Back to top

About this book

Aging is an almost universal process within biological systems, one which leads to a decline in functional capacity, disease onset, and eventually death. There has been much interest in recent years to elucidate the molecular mec- nisms that underlie the aging process. Many theories have been proposed since the last century that aim to explain the causes of aging. There is no one theory that completely satisfies the phenotype of aging, but genetics and environm- tal factors play an important role in the etiology of age-related pathologies and the aging process. However, there is still much to be learned about the aging process which has been termed one of the last great frontiers in biology. De- graphic changes worldwide are leading to increased average life expectancies within our populations. These changes in population characteristics will impact upon the economies of the supporting society, with increasing healthcare and infrastructural costs arising from the prevalence of age-related pathologies and other physical disabilities associated with advancing years. Many researchers worldwide are working in the attempt to identify key cellular processes through which it might one day be possible to slow down the aging process and thus increase the health span of humans. Numerous research projects—from the cellular through to tissue, organ, and whole organism studies—are currently underway to investigate the mul- factorial aging process.
Back to top

Reviews

"Aging Methods and Protocols brings together a wide variety of molecular experimental techniques for studying the aging process. A group of experts related their own aging experimental methods in a step-by-step manner. This format makes it easy to use and adapt specific techniques for individual laboratories. Aging Methods and Protocols contains a unique group of useful experimental methods. The book is highly recommended for academic research-oriented health science libraries. It is also recommended for individual research laboratories investigating the aging process." - E-STREAMS
Back to top

Editors and Affiliations

  • School of Biomedical Sciences, University of Ulster, Northern Ireland

    Yvonne A. Barnett, Christopher R. Barnett

Back to top

Bibliographic Information

  • Book Title: Aging Methods and Protocols

  • Editors: Yvonne A. Barnett, Christopher R. Barnett

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592590705

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-582-9Published: 26 May 2000

  • Softcover ISBN: 978-1-61737-083-0Published: 09 November 2010

  • eBook ISBN: 978-1-59259-070-4Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 385

  • Topics: Geriatrics/Gerontology

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation