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Chaperonin Protocols

  • Book
  • © 2000

Overview

Editors:
  1. Christine Schneider

    1. Max-Planck Institute for Biochemistry, Martinsried, Germany

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Part of the book series: Methods in Molecular Biology (MIMB, volume 140)

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Table of contents (22 protocols)

  1. Purification of Prefoldin

    • Sally A. Lewis, Nicholas J. Cowan
    Pages 179-184

  2. Purification of GimC from Saccharomyces cerevisiae

    • Katja Siegers, Elmar Schiebel
    Pages 185-193

  3. Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding

    • Michel R. Leroux
    Pages 195-206

  4. Back Matter

    Pages 207-212
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About this book

In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods-for example, one for preparing labeled probes-that can be used for various purposes and prove helpful in numerous different procedures.
Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results.

Reviews

"This book is highly recommended for academic libraries and for those special or industrial libraries that support a program in microbiology. Because the clear and logical approach to each protocol can serve as a model experiment that is easily reproduced, this book would be an especially valuable tool for students."-E-Streams (Electronic Reviews of Science and Technology References)

"...an invaluable overview of two main aspects of chaperonin research-purification of chaperonins and their cofactors and assays to monitor folding activity. ...of excellent chapters on more diverse chaperonins, including those from Archaea and the specialized, eukaryotic TRiC complex....The book provides a wealth of explicitly detailed protocols (together with useful troubleshooting notes) for all those working in the field of chaperonins and protein folding or misfolding."...Microbiology Today

Editors and Affiliations

  • Max-Planck Institute for Biochemistry, Martinsried, Germany

    Christine Schneider

Bibliographic Information

  • Book Title: Chaperonin Protocols

  • Editors: Christine Schneider

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590616

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-739-7Published: 19 January 2000

  • Softcover ISBN: 978-1-61737-163-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-061-2Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 212

  • Topics: Biochemistry, general

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