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  • © 2000

Chemokine Protocols

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Part of the book series: Methods in Molecular Biology (MIMB, volume 138)

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  • ISBN: 978-1-59259-058-2
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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xiii
  2. Chemokine Expression in Insect Cells

    • Toshio Imai
    Pages 23-32
  3. Expression of Chemokines in Escherichia coli

    • Michael D. Edgerton, Lars-Ole Gerlach, Thomas P. Boesen, Bernard Allet
    Pages 33-40
  4. Expression of Chemokines in the Periplasmic Space of E. coli

    • Jochen Pfirstinger, Matthias Mack
    Pages 41-45
  5. Synthesis of Chemokines

    • Ian Clark-Lewis
    Pages 47-63
  6. Identification of Novel Chemokines From Expressed Sequence Tag Databases

    • Timothy N. C. Wells, Manuel C. Peitsch
    Pages 65-73
  7. Purification of Recombinant Chemokines from E. coli

    • Amanda E. I. Proudfoot, Frédéric Borlat
    Pages 75-87
  8. Chemokine Receptor Cloning

    • Philip M. Murphy
    Pages 89-98
  9. Generation of Stable Cell Lines Expressing Chemokine Receptors

    • Christine A. Power, Alexandra Meyer
    Pages 99-104
  10. Modified Microchemotaxis Assays

    • Dennis D. Taub
    Pages 105-112
  11. Transwell Chemotaxis

    • Paul D. Ponath, Juan Wang, Heidi Heath
    Pages 113-120
  12. Endothelial Cell Chemotaxis Assays

    • Darcey Black
    Pages 121-127
  13. Radiolabeled Chemokine Binding Assays

    • Bruce L. Daugherty, Salvatore J. Siciliano, Martin S. Springer
    Pages 129-134
  14. Scintillation Proximity Binding Assay

    • Sami Alouani
    Pages 135-141
  15. Calcium Mobilization

    • Raphaële Buser, Amanda E. I. Proudfoot
    Pages 143-148
  16. Actin Polymerization

    • Jörn Elsner, Alexander Kapp
    Pages 149-152
  17. Reactive Oxygen Release

    • Jörn Elsner, Alexander Kapp
    Pages 153-156
  18. Histamine Release

    • Michael A. Lett-Brown, Rafeul Alam
    Pages 157-162

About this book

In the past decade research has established the biological importance of chemokines: they play a major role in leukocyte trafficking, in the recruitment of leukocytes to inflammatory sites, and are coreceptors along with CD4 for HIV cell entry. In Chemokine Protocols, expert investigators describe in detail important techniques usedchemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience.
Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.

Reviews

highly recommended...Seldom does on find so much valuable information, concisely presented and with such obvious potential for the future, made available in one volume." - E-Streams

"...Chemokine Protocols is a collection of clear, well written protocols and I would recommend it as an excellent starting point for any scientist with an interest in chemokines." - Molecular Biotechnology

Editors and Affiliations

  • Serono Pharmaceutical Research Institute, Geneva, Switzerland

    Amanda E. I. Proudfoot, Timothy N. C. Wells, Christine A. Power

Bibliographic Information

  • Book Title: Chemokine Protocols

  • Editors: Amanda E. I. Proudfoot, Timothy N. C. Wells, Christine A. Power

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590586

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-722-9

  • Softcover ISBN: 978-1-61737-151-6

  • eBook ISBN: 978-1-59259-058-2

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 353

  • Topics: Immunology

Buying options

eBook
USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-058-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 179.00
Price excludes VAT (USA)
Hardcover Book
USD 179.00
Price excludes VAT (USA)