DNA Topoisomerase Protocols

  • Neil Osheroff
  • Mary-Ann Bjornsti

Part of the Methods in Molecular Biology™ book series (MIMB, volume 95)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Lance Stewart, James J. Champoux
    Pages 1-11
  3. Andrea Haldane, Daniel M. Sullivan
    Pages 13-23
  4. Penny J. Sayer, Martin L. Goble, Mark Oram, L. Mark
    Pages 25-33
  5. Michel Duguet, Christine Jaxel, AnneCécile Déclais, Fabrice Confalonieri, Janine Marsault, Claire Bouthier de la Tour et al.
    Pages 35-49
  6. Paul S. Kingma, John M. Fortune, Neil Osheroff
    Pages 51-56
  7. Kenneth N. Kreuzer, Catherine H. Freudenreich, Yves Pommier
    Pages 89-99
  8. Anni H. Andersen, Kent Christiansen, Ole Westergaard
    Pages 101-117
  9. Thomas C. Rowe, Dale Grabowski, Ram Ganapathi
    Pages 129-135
  10. Deepa Subramanian, Christine Sommer Furbee, Mark T. Muller
    Pages 137-147
  11. David E. Graves
    Pages 161-169
  12. Thomas D. Gootz
    Pages 185-194
  13. Scott T. Taylor, Rolf Menzel
    Pages 195-204

About this book


DNA Topoisomerase Protocols, II: Enzymology and Drugs brings together an unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatenation, and ATP hydrolysis. Several modified DNA substrates, used to dissect enzyme mechanisms by trapping intermediates, are also described. Methodologies to determine the action of topoisomerase-targeted drugs include biochemical assays of drug-induced enzyme-DNA complexes, methods for assaying drug uptake, and cell-based assays for determining drug specificity and mechanisms of resistance. A companion volume, DNA Topoisomerase Protocols, I: DNA Topology and Enzymes, provides state-of-the-art experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function.

DNA Topoisomerase Protocols, II: Enzymology and Drugs provides, for the first time, a comprehensive description of the methods and experimental systems needed to investigate fully the catalytic mechanisms of DNA topoisomerases from bacterial, viral, and eukaryotic sources. For the many basic scientists and clinicians who want better to understand and to exploit the action of topoisomerase-targeted drugs in a variety of in vitro and in vivo systems, it will immediately develop immense value as the new standard reference in the field.

Editors and affiliations

  • Neil Osheroff
    • 1
  • Mary-Ann Bjornsti
    • 2
  1. 1.Vanderbilt University School of MedicineNashville
  2. 2.St. Jude Children’s Research HospitalMemphis

Bibliographic information

  • DOI https://doi.org/10.1385/1592590578
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-512-6
  • Online ISBN 978-1-59259-057-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book