Calpain Methods and Protocols

  • John S. Elce

Part of the Methods in Molecular Biology™ book series (MIMB, volume 144)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Purification of Components of the Calpain System

    1. Front Matter
      Pages 1-105
    2. Valery F Thompson, Darrel E Goll
      Pages 1-16
    3. Gàspàr Jékely, Marianna Pintér, Peter Friedrich
      Pages 67-74
    4. Masatoshi Maki, Kiyotaka Hitomi
      Pages 85-94
    5. Masatoshi Maki, Kiyotaka Hitomi
      Pages 95-98
    6. Edon Melloni, Mauro Michetti, Franca Salamino, Roberto Minafra, Bianca Sparatore, Sandro Pontremoli
      Pages 99-105
  3. Assays of Calpain

    1. Front Matter
      Pages 109-169
    2. J. Simon C. Arthur, Donald L. Mykles
      Pages 109-116
    3. Xiurong Zhao, Jennifer K. Newcomb, Brian R. Pike, Ronald L. Hayes
      Pages 117-120
    4. J. Simon C. Arthur, John S. Elce
      Pages 121-127
    5. Peter Tompa, Peter Friedrich
      Pages 129-136
    6. Peter Tompa, Éva Schád, Peter Friedrich
      Pages 137-141

About this book

Introduction

In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of µ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains.

Editors and affiliations

  • John S. Elce
    • 1
  1. 1.Department of BiochemistryQueen’s UniversityKingstonCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592590500
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-632-1
  • Online ISBN 978-1-59259-050-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book