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Neuropeptide Y Protocols

  • Ambikaipakan Balasubramaniam

Part of the Methods in Molecular Biology™ book series (MIMB, volume 153)

Table of contents

  1. Front Matter
    Pages i-x
  2. Cloning Techniques

    1. Front Matter
      Pages 1-1
    2. Carolyn A. Worby
      Pages 3-11
    3. Herbert Herzog
      Pages 13-24
    4. David A. Ammar, Debra A. Thompson
      Pages 25-43
    5. Ingrid Lundell, Magnus M. Berglund, Dan Larhammar
      Pages 45-51
    6. Eric M. Parker
      Pages 53-60
    7. Douglas J. MacNeil, David H. Weinberg
      Pages 61-70
  3. Production of Transgenic and Knock-Out Models

    1. Front Matter
      Pages 71-71
    2. Mieczyslaw Michalkiewicz, Teresa Michalkiewicz
      Pages 73-89
    3. Thierry Pedrazzini, Josiane Seydoux
      Pages 91-100
  4. Antisense Technology

    1. Front Matter
      Pages 101-101
    2. Sulaiman Sheriff, William T. Chance, Ambikaipakan Balasubramaniam
      Pages 103-114
    3. Thierry Voisin, Anne-Marie Lorinet, Mathieu Goumain, Marc Laburthe
      Pages 115-128
    4. Andrea O. Schaffhauser, Alain Stricker-Krongrad, Karl G. Hofbauer
      Pages 129-150
    5. Pushpa S. Kalra, Satya P. Kalra
      Pages 151-161
  5. Techniques to Study Tissue Distribution of mRNA

    1. Front Matter
      Pages 163-163
    2. Philip J. Larsen, Peter Kristensen
      Pages 185-198
    3. Jennifer Lachey, Jennifer Profitt, Chad Foradori, Randy J. Seeley
      Pages 199-206
  6. Miscellaneous Techniques

    1. Front Matter
      Pages 207-207
    2. Mathieu Goumain, Thierry Voisin, Marc Laburthe
      Pages 209-217
    3. Karen E. McCrea, Herbert Herzog
      Pages 231-239
  7. Back Matter
    Pages 241-246

About this book

Introduction

It is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic opportunities for their treatment. In Neuropeptide Y Protocols, a strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal transduction pathways mediating NPY actions. A range of the major qualitative and quantitative techniques used to study tissue mRNA distribution are also included, as well as methods for radioligand binding. The cloning techniques utilize genomic DNA isolation, expression cloning, PCR cloning, the construction of hybrid receptors, and homology-based cloning.
Highly practical and state-of-the-art, Neuropeptide Y Protocols constitutes a gold-standard collection of proven techniques for advancing today's understanding of this significant neuropeptide, as well as for developing the promising NPY-based therapeutics now emerging for many important disorders.

Editors and affiliations

  • Ambikaipakan Balasubramaniam
    • 1
  1. 1.Department of SurgeryUniversity of Cincinnati Medical CenterCincinnati

Bibliographic information

  • DOI https://doi.org/10.1385/159259042X
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-662-8
  • Online ISBN 978-1-59259-042-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site