Receptor Signal Transduction Protocols

  • R. A. J. Challiss

Part of the Methods in Molecular Biology™ book series (MIMB, volume 83)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Methods for Characterizing Receptors

  3. Methods for Studying Receptor Coupling Mechanisms

  4. Methods for Investigating the Regulation of Receptor Function

About this book

Introduction

R. A. J. Challiss assembles in Receptor Signal Transduction Protocols an outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. These powerful methods emphasize the regulation of G-protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G-proteins, and approaches to studying how receptor expression and function is regulated. Designed to be easily reproducible, the protocols offered include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G-protein function, antisense methods for defining receptor-effector coupling pathways, and many more.

Receptor Signal Transduction Protocols blends authoritative descriptions and applications of well-established methodologies with many novel techniques at the cutting edge of signal transduction research. The coverage cuts across disciplines and allows those already well-versed in one area to expand their repertoire of potent methods and broaden their research horizons. Every researcher interested in signal transduction today will find this richly practical guide indispensable.

Editors and affiliations

  • R. A. J. Challiss
    • 1
  1. 1.University of LeicesterUK

Bibliographic information

  • DOI https://doi.org/10.1385/089603495X
  • Copyright Information Humana Press 1997
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-495-2
  • Online ISBN 978-1-59259-567-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book