Free Radical and Antioxidant Protocols

  • Donald Armstrong

Part of the Methods in Molecular Biology™ book series (MIMB, volume 108)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Techniques for the Measurement of Oxidative Stress

    1. Front Matter
      Pages 1-1
    2. Istvan Stadler
      Pages 3-14
    3. Daniel J. Brackett, Gemma Wallis, Michael F. Wilson, Paul B. McCay
      Pages 15-25
    4. Yoshinori Mizuguchi
      Pages 37-55
    5. Andrea Ghiselli
      Pages 89-100
    6. Yves Chancerelle, J. Mathieu, J. F. Kergonou
      Pages 111-118
    7. Jan Galle, Christoph Wanner
      Pages 119-130
    8. Odile Sergent, Josiane Cillard
      Pages 131-137
    9. Donald Armstrong, Richard W. Browne
      Pages 139-145
    10. Kazumasa Aoyagi, Sohji Nagase, Akio Koyama, Mitsuharu Narita, Shizuo Tojo
      Pages 157-164
    11. Thomas M. Wengenack, J. Randall Slemmon, J. Mark Ordy
      Pages 165-180

About this book


In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications.

Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.

Editors and affiliations

  • Donald Armstrong
    • 1
  1. 1.State University of New YorkBuffalo

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-472-3
  • Online ISBN 978-1-59259-254-8
  • Series Print ISSN 1064-3745
  • About this book