Basic DNA and RNA Protocols Editors (view affiliations) Adrian J. Harwood Book 23 Citations 526 Readers 1 Reviews 319k Downloads Part of the Methods in Molecular Biology™ book series (MIMB, volume 58)
Protocols Table of contents (58 protocols) About About this book Table of contents Page 1 Navigate to page number of 4 Next Navigate to page number Search within book Front Matter Pages i-xiv PDF DNA Analysis Front Matter Pages 1-1 PDF The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau Pages 3-9 Restriction Endonuclease Digestion of DNA Duncan R. Smith Pages 11-15 Agarose Gel Electrophoresis Duncan R. Smith Pages 17-21 Capillary Blotting of Agarose Gels Duncan R. Smith, David Murphy Pages 23-25 Random Primed 32P-Labeling of DNA Duncan R. Smith Pages 27-29 Hybridization and Competition Hybridization of Southern Blots Rosemary E. Kelsell Pages 31-39 Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations Tim Helentjaris, Tom McCreery Pages 41-51 The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence Martin Cunningham, Martin Harris Pages 53-63 Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay Martin Cunningham Pages 65-66 The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence Bronwen Harvey, Ian Durrant, Martin Cunningham Pages 67-75 3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection Martin Cunningham, Ian Durrant, Bronwen Harvey Pages 77-82 The Preparation of Riboprobes Dominique Belin Pages 83-91 Native Polyacrylamide Gel Electrophoresis Adrian J. Harwood Pages 93-96 Use of Silver Staining to Detect Nucleic Acids Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril Pages 97-103 End-Labeling of DNA Fragments Adrian J. Harwood Pages 105-110 RNA Analysis Northern Blot Analysis Robb Krumlauf Pages 113-128 RNA Slot Blotting David Murphy Pages 129-130 The RNase Protection Assay Dominique Belin Pages 131-136 Page 1 Navigate to page number of 4 Next About this book Editors and affiliations Adrian J. Harwood11.MRC Laboratory for Molecular Cell BiologyLondonUK Bibliographic information DOI https://doi.org/10.1385/089603402X Copyright Information Humana Press 1996 Publisher Name Humana Press eBook Packages Springer Protocols Print ISBN 978-0-89603-402-0 Online ISBN 978-1-59259-251-7 Series Print ISSN 1064-3745 Series Online ISSN 1940-6029 About this book Buy options