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Basic DNA and RNA Protocols

  • Adrian J. Harwood
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 58)

Table of contents

  1. Front Matter
    Pages i-xiv
    PDF

  2. DNA Analysis

    1. Front Matter
      Pages 1-1
      PDF
    2. The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells
      Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau
      Pages 3-9
    3. Restriction Endonuclease Digestion of DNA
      Duncan R. Smith
      Pages 11-15
    4. Agarose Gel Electrophoresis
      Duncan R. Smith
      Pages 17-21
    5. Capillary Blotting of Agarose Gels
      Duncan R. Smith, David Murphy
      Pages 23-25
    6. Random Primed 32P-Labeling of DNA
      Duncan R. Smith
      Pages 27-29
    7. Hybridization and Competition Hybridization of Southern Blots
      Rosemary E. Kelsell
      Pages 31-39
    8. Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations
      Tim Helentjaris, Tom McCreery
      Pages 41-51
    9. The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence
      Martin Cunningham, Martin Harris
      Pages 53-63
    10. Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay
      Martin Cunningham
      Pages 65-66
    11. The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence
      Bronwen Harvey, Ian Durrant, Martin Cunningham
      Pages 67-75
    12. 3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
      Martin Cunningham, Ian Durrant, Bronwen Harvey
      Pages 77-82
    13. The Preparation of Riboprobes
      Dominique Belin
      Pages 83-91
    14. Native Polyacrylamide Gel Electrophoresis
      Adrian J. Harwood
      Pages 93-96
    15. Use of Silver Staining to Detect Nucleic Acids
      Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril
      Pages 97-103
    16. End-Labeling of DNA Fragments
      Adrian J. Harwood
      Pages 105-110

  3. RNA Analysis

    1. Northern Blot Analysis
      Robb Krumlauf
      Pages 113-128
    2. RNA Slot Blotting
      David Murphy
      Pages 129-130
    3. The RNase Protection Assay
      Dominique Belin
      Pages 131-136
    4. Primer Extension Analysis of mRNA
      Mark W. Leonard, Roger K. Patient
      Pages 137-145
    5. S1 Mapping Using Single-Stranded DNA Probes
      Stéphane Viville, Roberto Mantovani
      Pages 147-153
    6. Nonradioactive In Situ Hybridization for Cells and Tissues
      Ian Durrant
      Pages 155-167

  4. Gene Cloning

    1. In Vitro Packaging of DNA
      Jeremy W. Dale, Peter J. Greenaway
      Pages 171-175
    2. Construction of Mammalian Genomic Libraries Using λ Replacement Vectors
      Alan N. Bateson, Jeffrey W. Pollard
      Pages 177-190
    3. The Production of Double-Stranded Complementary DNA for Use in Making Libraries
      Steve Mayall, Jane Kirk
      Pages 191-198
    4. Construction of cDNA Libraries
      Michael M. Burrell
      Pages 199-209
    5. Screening λ Libraries
      Janet C. Harwood, Adrian J. Harwood
      Pages 211-218

  5. Subcloning Methods

    1. Subcloning Strategies and Protocols
      Danielle Gioioso Taghian, Jac A. Nickoloff
      Pages 221-235
    2. Purification of DNA Fragments from Agarose Gels Using Glass Beads
      Etienne Joly
      Pages 237-240
    3. Transformation of E. coli
      Fiona M. Tomley
      Pages 241-247
    4. Transformation of Bacteria by Electroporation
      Lucy Drury
      Pages 249-256
    5. Preparation of Plasmid DNA by Alkaline Lysis
      Etienne Joly
      Pages 257-263
    6. The Rapid Boiling Method for Small-Scale Preparation of Plasmid DNA
      Adrian J. Harwood
      Pages 265-267
    7. Plasmid Preparations with Diatomaceous Earth
      Laura Machesky
      Pages 269-272

  6. PCR Techniques

    1. Polymerase Chain Reaction
      Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White
      Pages 275-292
    2. Inverse Polymerase Chain Reaction
      Daniel L. Hartl, Howard Ochman
      Pages 293-301
    3. Polymerase Chain Reaction with Degenerate Oligonucleotide Primers to Clone Gene Family Members
      Gregory M. Preston
      Pages 303-312
    4. Cloning PCR Products Using T-Vectors
      Michael K. Trower, Greg S. Elgar
      Pages 313-324
    5. Direct Radioactive Labeling of Polymerase Chain Reaction Products
      Tim McDaniel, Stephen J. Meltzer
      Pages 325-327
    6. A Rapid PCR-Based Colony Screening Protocol for Cloned Inserts
      Michael K. Trower
      Pages 329-333
    7. Use of Polymerase Chain Reaction to Screen Phage Libraries
      Lei Yu, Laura J. Bloem
      Pages 335-339

  7. DNA Sequencing

    1. Cloning into M13 Bacteriophage Vectors
      Qingzhong Yu
      Pages 343-348
    2. Ordered Deletions Using Exonuclease III
      Denise Clark, Steven Henikoff
      Pages 349-357
    3. M13 Phage Growth and Single-Stranded DNA Preparation
      Fiona M. Tomley
      Pages 359-362
    4. Preparation of ssDNA from Phagemid Vectors
      Michael K. Trower
      Pages 363-366
    5. A Rapid Plasmid Purification Method for Dideoxy Sequencing
      Annette M. Grifln, Hugh G. Grifln
      Pages 367-371
    6. DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase
      Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin
      Pages 373-387
    7. Pouring Linear and Buffer-Gradient Sequencing Gels
      Paul Littlebury
      Pages 389-392
    8. Electrophoresis of Sequence Reaction Samples
      Alan T. Bankier
      Pages 393-401

About this book

Editors and affiliations

  • Adrian J. Harwood
    • 1
  1. 1.MRC Laboratory for Molecular Cell BiologyLondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/089603402X
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-402-0
  • Online ISBN 978-1-59259-251-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site