Basic DNA and RNA Protocols

  • Adrian J. Harwood
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 58)

Table of contents

  1. Front Matter
    Pages i-xiv
    PDF

  2. DNA Analysis

    1. Front Matter
      Pages 1-1
      PDF
    2. The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells
      Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau
      Pages 3-9
    3. Restriction Endonuclease Digestion of DNA
      Duncan R. Smith
      Pages 11-15
    4. Agarose Gel Electrophoresis
      Duncan R. Smith
      Pages 17-21
    5. Capillary Blotting of Agarose Gels
      Duncan R. Smith, David Murphy
      Pages 23-25
    6. Random Primed 32P-Labeling of DNA
      Duncan R. Smith
      Pages 27-29
    7. Hybridization and Competition Hybridization of Southern Blots
      Rosemary E. Kelsell
      Pages 31-39
    8. Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations
      Tim Helentjaris, Tom McCreery
      Pages 41-51
    9. The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence
      Martin Cunningham, Martin Harris
      Pages 53-63
    10. Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay
      Martin Cunningham
      Pages 65-66
    11. The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence
      Bronwen Harvey, Ian Durrant, Martin Cunningham
      Pages 67-75
    12. 3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection
      Martin Cunningham, Ian Durrant, Bronwen Harvey
      Pages 77-82
    13. The Preparation of Riboprobes
      Dominique Belin
      Pages 83-91
    14. Native Polyacrylamide Gel Electrophoresis
      Adrian J. Harwood
      Pages 93-96
    15. Use of Silver Staining to Detect Nucleic Acids
      Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril
      Pages 97-103
    16. End-Labeling of DNA Fragments
      Adrian J. Harwood
      Pages 105-110

  3. RNA Analysis

    1. Northern Blot Analysis
      Robb Krumlauf
      Pages 113-128
    2. RNA Slot Blotting
      David Murphy
      Pages 129-130
    3. The RNase Protection Assay
      Dominique Belin
      Pages 131-136

About this book

Editors and affiliations

  • Adrian J. Harwood
    • 1
  1. 1.MRC Laboratory for Molecular Cell BiologyLondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/089603402X
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-402-0
  • Online ISBN 978-1-59259-251-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book