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Protein Synthesis

Methods and Protocols

  • Robin Martin

Part of the Methods in Molecular Biology book series (MIMB, volume 77)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Stephen Douthwaite
    Pages 11-21
  3. Romualdas Stapulionis, Murray P. Deutscher
    Pages 23-31
  4. Jon S. Thorson, Virginia W. Cornish, Jeannie E. Barrett, Sharon T. Cload, Takato Yano, Peter G. Schultz
    Pages 43-73
  5. Obed W. Odom, Wieslaw Kudlicki, Boyd Hardesty
    Pages 93-103
  6. Marcel F. Brink, Herman A. de Boer
    Pages 117-128
  7. Michael A. Sørensen, Steen Pedersen
    Pages 129-142
  8. Bernard N. Violand, Gregg Bogosian
    Pages 143-151
  9. Margaret J. Hollingsworth, Jeong-Kook Kim, Neil E. Stollar
    Pages 153-165
  10. Thomas E. Dever
    Pages 167-178
  11. Lyubov A. Ryabova, lgor Yu. Morozov, Alexander S. Spirin
    Pages 179-193
  12. Virginia M. Pain, Tina D. Patrick, Richard Cox, Simon J. Morley
    Pages 195-209
  13. Michael Rau, Theophile Ohlmann, Virginia M. Pain, Simon J. Morley
    Pages 211-226
  14. Neş’e Bilgin, Måns Ehrenberg
    Pages 243-256
  15. Jaap Venema, Rudi J. Planta, Hendrik A. Raué
    Pages 257-270
  16. Steven T. Gregory, Carleen A. Brunelli, J. Stephen Lodmell, Michael O’Connor, Albert E. Dahlberg
    Pages 271-281
  17. Steven Ringquist, Larry Gold
    Pages 283-295
  18. Andrey V. Bakin, James Ofengand
    Pages 297-309
  19. Cristina Rodriguez-Fonseca, Roger A. Garrett
    Pages 311-323
  20. Lance E. Steward, A. Richard Chamberlin
    Pages 325-354
  21. Chang-you Chen, Peter Sarnow
    Pages 355-363
  22. Arunachalam Vimaladithan, Philip J. Farabaugh
    Pages 399-411
  23. Back Matter
    Pages 427-442

About this book

Introduction

The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca­ tions is arguably the most complex task performed by cells. Protein Synthesis collects together methods and protocols covering a range of different approaches towards understanding how the cellular machinery accomplishes this task and how these ftinctions might be harnessed by the biotechnology industry to generate novel and useful proteins. The era in which the components of the translational machinery were being catalogued is over. This volume gathers together protocols that focus on preserving and describing the dynamic function as closely as possible. The need to understand exactly how ribosomes are positioned on messages or where tRNA molecules, translation factors, or control proteins are bound, has been appreciated by many of the authors. Several chapters that explore the fidelity and processivity of translation reflect this belief. Moreover, the fundamental importance of rRNA at the heart of the ribosome is a strong theme in a number of the protocols. These articles include in vitro and in vivo systems from bacterial, fungal, plant, and animal systems. Overall, Protein Synthesis might be characterized by the novelty of the approaches employed to illuminate the inner workings of the protein synthetic machinery as well as by the inventiveness of the attempts to harness these reactions for biotechnological applications.

Editors and affiliations

  • Robin Martin
    • 1
  1. 1.Blond McIndoe CenterQueen Victoria HospitalSussex, England

Bibliographic information

  • DOI https://doi.org/10.1385/089603397X
  • Copyright Information Humana Press 1998
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-397-9
  • Online ISBN 978-1-59259-563-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site