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Neurotransmitter Methods

  • Richard C. Rayne

Part of the Methods in Molecular Biology book series (MIMB, volume 72)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ting Wang, Ira S. Kass
    Pages 1-14
  3. Peter J. Bergold, Patrizia Casaccia-Bonnefil
    Pages 15-22
  4. Alexandra I. M. Breukel, Elly Besselsen, Wim E. J. M. Ghijsen
    Pages 33-47
  5. Miriam N. G. Titulaer, Wim E. J. M. Ghijsen
    Pages 49-59
  6. Dick R. Nässel, Peter Ekström
    Pages 71-101
  7. Jan de Vente, Harry W. M. Steinbusch
    Pages 125-143
  8. Suzanna J. Newman, Stephen M. Gentleman
    Pages 145-152
  9. Sophie Sarre, Katrien Thorré, Ilse Smolders, Yvette Michotte
    Pages 185-196
  10. Ilse Smolders, Sophie Sarre, Guy Ebinger, Yvette Michotte
    Pages 197-204
  11. G. Mark Holman, Timothy K. Hayes
    Pages 205-218
  12. David G. Watson, Richard A. Baines, John M. Midgley, Jonathan P. Bacon
    Pages 225-237
  13. Back Matter
    Pages 267-271

About this book

Introduction

Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action. Each method is detailed in a user-friendly "recipe" format and the protocols are accompanied by extensive notes to high­ light and explain crucial steps. Approaches utilizing an incredibly diverse array of modem techniques are presented: methods including HPLC, histochemistry, immunocytochemistry, in situ hybridization, mass spectrometry, microdialysis, and electrochemistry all make at least one appearance. In addition, protocols for associated methodologies, including the production of brain slices, dissociated neurons, synaptosomes/synaptoneurosomes, and neuronal plasma membranes are presented. Methods applicable to most of the recognized chemical types of neu­ rotransmitter are to be found and, although you may find absent any mention of your favorite neurotransmitter, many of the protocols are sufficiently general to be adapted to alternative uses. So, how does this book contribute usefiilly to the horde of methods volumes stampeding across our bookshelves? 1 hope that the strength oiNeurotransmitter Methods lies in the variety of its content. The book provides in a single volume an array of techniques that could take a researcher from selection and preparation of a tissue source through to identification and measurement of neurotransmitter content and even onto characterization of neurotransmitter sites of action. Furthermore, the diversity of approaches presented reflects the need for today's bench neuroscientist to be a bit of a "jack (or a "jill"!) of all trades.

Editors and affiliations

  • Richard C. Rayne
    • 1
  1. 1.Birkbeck CollegeLondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033945
  • Copyright Information Humana Press 1997
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-394-8
  • Online ISBN 978-1-59259-558-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site