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PCR Sequencing Protocols

  • Ralph Rapley

Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Bimal D. M. Theophilus
    Pages 1-9
  3. Frank C. Brosius III, Lawrence B. Holzman, Xinan Cao
    Pages 11-21
  4. Georges-Raoul Mazars, Charles Theillet
    Pages 35-40
  5. Bentley A. Atchison, Andrea M. Douglas
    Pages 49-55
  6. Joakim Lundeberg, Bertil Pettersson, Mathias Uhlén
    Pages 57-66
  7. Anu Suomalainen, Ann-Christine Syvänen
    Pages 67-72
  8. Anu Suomalainen, Ann-Christine Syvänen
    Pages 73-79
  9. Siegfried Kösel, Christoph B. Lücking, Rupert Egensperger, Manuel B. Graeber
    Pages 81-89
  10. Alison Wade-Evans
    Pages 91-100
  11. Harald Petry, Barbara Bachmann, Wolfgang Lüke, Gerhard Hunsmann
    Pages 105-109
  12. Zhiyuan Shen, Jingmei Liu, Robert L. Wells, Mortimer M. Elkind
    Pages 111-118
  13. Jean-Pierre Quivy, Peter B. Becker
    Pages 119-131
  14. Eran Pichersky
    Pages 133-136
  15. Wei Zhang, Albert B.
    Pages 137-147
  16. Bernhard Kaltenboeck, Konstantin G. Kousoulas
    Pages 149-153
  17. Mary I. Coolbaugh Murphy, Holly A. Hammond, C. Thomas Caskey
    Pages 163-176
  18. Tammy Lind, Erik C. Thorland, Steve S. Sommer
    Pages 193-200
  19. Marcia A. McAleer, Alison Coffey, Ian Dunham
    Pages 201-208
  20. Barbara Anne Hales, Craig Winstanley
    Pages 209-218
  21. Back Matter
    Pages 219-221

About this book

Introduction

Advances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Editors and affiliations

  • Ralph Rapley
    • 1
  1. 1.School of Natural SciencesCoventry UniversityCoventryUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033449
  • Copyright Information Humana Press 1996
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-344-3
  • Online ISBN 978-1-59259-551-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site