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Plant Cell Electroporation and Electrofusion Protocols

  • Jac A. Nickoloff

Part of the Methods in Molecular Biology™ book series (MIMB, volume 55)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Theory and Instrumentation

    1. James C. Weaver
      Pages 3-28
    2. Gunter A. Hofmann
      Pages 41-59
  3. Electroporation Protocols

    1. Amke den Dulk-Ras, Paul J. J. Hooykaas
      Pages 63-72
    2. James A. Saunders, Benjamin F. Matthews
      Pages 81-88
    3. Patrick Gallois, Keith Lindsey, Renee Malone
      Pages 89-107
    4. Frank Siegemund, Klaus Eimert
      Pages 109-120
    5. Carol A. Rhodes, Kathleen A. Marrs, Lynn E. Murry
      Pages 121-131
    6. Kathleen A. Marrs, J. C. Carle Urioste
      Pages 133-145
    7. Benjamin F. Matthews, James A. Saunders, Joan S. Gebhardt, Jhy-Jhu Lin, Susan M. Koehler
      Pages 147-162
  4. Electrofusion Protocols

    1. Harold N. Trick, George W. Bates
      Pages 165-179
    2. Jianping Cheng, James A. Saunders
      Pages 181-188
  5. Back Matter
    Pages 203-205

About this book

Introduction

Gene transfer is an essential technology for improving our under­ standing of gene structure and function. Although there are many meth­ ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi­ ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in­ cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna­ tives to electric field-based methods, citing the advantages and limita­ tions of the various methods for achieving specific goals.

Editors and affiliations

  • Jac A. Nickoloff
    • 1
  1. 1.Harvard UniversityBoston

Bibliographic information

  • DOI https://doi.org/10.1385/0896033287
  • Copyright Information Humana Press 1995
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-328-3
  • Online ISBN 978-1-59259-542-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site