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Capillary Electrophoresis Guidebook

Principles, Operation, and Applications

  • Kevin D. Altria

Part of the Methods in Molecular Biology book series (MIMB, volume 52)

Table of contents

  1. Front Matter
    Pages i-ix
  2. General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation

    1. Kevin D. Altria
      Pages 15-19
    2. Kevin D. Altria
      Pages 21-28
    3. Kevin D. Altria
      Pages 29-48
    4. Kevin D. Altria
      Pages 49-60
    5. Kevin D. Altria
      Pages 71-81
    6. Kevin D. Altria
      Pages 83-97
    7. Kevin D. Altria
      Pages 99-103
    8. Kevin D. Altria
      Pages 105-118
    9. Kevin D. Altria
      Pages 119-122
  3. Applications of Capillary Electrophoresis and Specific Technologies

    1. Koji Otsuka, Shigeru Terabe
      Pages 125-155
    2. András Guttman
      Pages 157-169
    3. Manus M. Rogan, Kevin D. Altria
      Pages 171-196
    4. Iain H. Grant
      Pages 197-209
    5. Dean S. Burgi, Ring-Ling Chien
      Pages 211-226
    6. Marzell Herold, Gordon A. Ross, Rudolf Grimm, David N. Heiger
      Pages 285-308
  4. Back Matter
    Pages 345-349

About this book

Introduction

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro­ vided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth­ ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

Editors and affiliations

  • Kevin D. Altria
    • 1
  1. 1.Glaxo Research and DevelopmentWare, HertfordshireUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033155
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-315-3
  • Online ISBN 978-1-59259-539-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site