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Animal Cell Electroporation and Electrofusion Protocols

  • Jac A. Nickoloff

Part of the Methods in Molecular Biology book series (MIMB, volume 48)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Theory and Instrumentation

    1. James C. Weaver
      Pages 3-28
    2. Gunter A. Hofmann
      Pages 41-59
  3. Electroporation Protocols

    1. William F. Morgan, Joseph P. Day
      Pages 63-71
    2. Paul L. Campbell, James McCluskey, Jing Ping Yeo, Ban-Hock Toh
      Pages 83-92
    3. Leda H. Raptis, Kevin L. Firth, Heather L. Brownell, Andrea Todd, W. Craig Simon, Brian M. Bennett et al.
      Pages 93-113
    4. Danielle Gioioso Taghian, Jac A. Nickoloff
      Pages 115-121
    5. Ruth H. Paulssen, Eyvind J. Paulssen, Kaare M. Gautvik
      Pages 123-131
    6. Fen Xia, Howard L. Liber
      Pages 151-160
    7. Marc Gagné, François Pothier, Marc-André Sirard
      Pages 161-166
    8. Maurice J. B. van den Hoff, Vincent M. Christoffels, Wil T. Labruyère, Antoon F. M. Moorman, Wouter H. Lamers
      Pages 185-197
    9. Djenann Saint-Dic, Michael S. DuBow
      Pages 199-210
    10. Michael K. Showe, Louise C. Showe
      Pages 211-224
    11. K. Puloma Kamdar, Thao N. Wagner, Victoria Finnerty
      Pages 239-243
    12. Koji Inoue, Jun-ichiro Hata, Shinya Yamashita
      Pages 245-251
    13. Leslie Tung
      Pages 253-271
    14. Kathryn E. Matthews, Sukhendu B. Dev, Frances Toneguzzo, Armand Keating
      Pages 273-280
  4. Electrofusion Protocols

    1. Kenneth L. White
      Pages 283-293
    2. Josef Fulka Jr., Robert M. Moor, Josef Fulka
      Pages 309-316
    3. Akira Iritani, Tasuku Mitani
      Pages 317-329
    4. Mark J. Jaroszeski, Richard Gilbert, Richard Heller
      Pages 355-363
  5. Back Matter
    Pages 365-369

About this book

Introduction

The ability to introduce macromolecules into animal cells, includ­ ing DNA, RNA, proteins, and other bioactive compounds has facili­ tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud­ ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor­ tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter­ est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.

Editors and affiliations

  • Jac A. Nickoloff
    • 1
  1. 1.Department of Cancer BiologyHarvard University School of Public HealthBoston

Bibliographic information

  • DOI https://doi.org/10.1385/089603304X
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-304-7
  • Online ISBN 978-1-59259-535-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site