In Vitro Transcription and Translation Protocols

  • Martin J. Tymms

Part of the Methods in Molecular Biology book series (MIMB, volume 37)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Clifford W. Schweinfest, Peter S. Nelson, Michael W. Graber, Rita I. Demopoulos, Takis S. Papas
    Pages 13-30
  3. Beqjamin J. Blencowe, Silvia M. L. Barabino
    Pages 67-76
  4. Jobst Greeve, James Scott
    Pages 77-87
  5. Kai Tiller, Gerhard Link
    Pages 121-133
  6. Aldo Ceriotti, Alan Colman
    Pages 151-178
  7. Brigitte Kruse, Nalini N. Murdter, Giuseppe Attardi
    Pages 179-197
  8. Glenn M. Matthews, Alan Colman
    Pages 199-213
  9. Gregory S. Beckler, David Thompson, Tom Van Oosbree
    Pages 215-232
  10. Hiroshi Sakurai, Susumu Izumi, Shiro Tomino
    Pages 233-244
  11. John F Van Herwynen, Gregory S. Beckler
    Pages 245-251
  12. Scott A. Lesley
    Pages 265-278

About this book

Introduction

Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.

Editors and affiliations

  • Martin J. Tymms
    • 1
  1. 1.Monash University ClaytonVictoriaAustralia

Bibliographic information

  • DOI https://doi.org/10.1385/0896032884
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-288-0
  • Online ISBN 978-1-59259-524-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book