Protocols for Oligonucleotides and Analogs

1. Front Matter

   Pages i-xiv

   PDF

2. A Brief History of Oligonucleotide Synthesis

   • Daniel M. Brown

   Pages 1-17

3. Oligonucleotide Synthesis

   • Chris Christodoulou

   Pages 19-31

4. Oligodeoxyribonucleotides Synthesis

   • Serge L. Beaucage

   Pages 33-61

5. Oligodeoxynucleotide Synthesis

   • Brian C. Froehler

   Pages 63-80

6. Oligoribonucleotide Synthesis

   • Masad J. Damha, Kelvin K Ogilvie

   Pages 81-114

7. Synthesis of 2′-O-Alkyloligoribonucleotides

   • Brian S. Sproat

   Pages 115-141

8. An Improved Method for the Synthesis and Deprotection of Methylphosphonate Oligonucleotides

   • Richard I. Hogrefe, Mark A. Reynolds, Morteza M. Vaghefi, Kevin M. Young, Timothy A. Riley, Robert E. Klem et al.

   Pages 143-164

9. Oligonucleoside Phosphorothioates

   • Gerald Zon

   Pages 165-189

10. Synthesis and Purification of Phosphorodithioate DNA

    • W. T. Wiesler, W. S. Marshall, M. H. Caruthers

    Pages 191-206

11. Oligodeoxyribonucleotide Phosphotriesters

    • Maria Koziolkiewicz, Andrzej Wilk

    Pages 207-224

12. Oligonucleoside Boranophosphate (Borane Phosphonate)

    • Barbara Ramsay Shaw, Jon Madison, Anup Sood, Bernard F. Spielvogel

    Pages 225-243

13. Oligonucleotide Phosphorofluoridates and Fluoridites

    • Wojciech Dabkowski, Jan Michahki, Friedrich Cramer

    Pages 245-260

14. α-Oligodeoxynucleotides

    • François Morvan, Bernard Rayner, Jean-Louis Imbach

    Pages 261-283

15. Stereospecific Synthesis of P-Chiral Analogs of Oligonucleotides

    • Wojciech J. Stec, Zbigniew J. Lesnikowski

    Pages 285-313

16. Oligonucleotide Analogs with Dimethylenesulfide, -sulfoxide, and -sulfone Groups Replacing Phosphodiester Linkages

    • Zhen Huang, K. Christian Schneider, Steven A. Benner

    Pages 315-353

17. Oligonucleotide Analogs Containing Dephospho-Internucleoside Linkages

    • Eugen Uhlmann, Anusch Peyman

    Pages 355-389

18. Scale-Up of Oligonucleotide Synthesis

    • H. Seliger

    Pages 391-435

19. Large-Scale Oligonucleotide Synthesis Using the Solid-Phase Approach

    • Nanda D. Sinha

    Pages 437-463

20. Solid-Phase Supports for Oligonucleotide Synthesis

    • Richard T. Pon

    Pages 465-496

When first conceived, not only was the aim of Protocols for Oligo­ nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe­ sizers has become a common practice in numerous laboratories. How­ ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle­ otide analogs have been synthesized and studied for their individual prop­ erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo­ nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.

...comprehensive and authoritative definitely required reading for the would-be oligonucleotide chemist and for those involved in biological applications of oligonucleotides... - Journal of Pharmacy and Pharmacology

• Worcester Foundation for Experimental Biology, Shrewsbury

  Sudhir Agrawal

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