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Protocols for Oligonucleotides and Analogs

Synthesis and Properties

  • Sudhir Agrawal

Part of the Methods in Molecular Biology book series (MIMB, volume 20)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Chris Christodoulou
    Pages 19-31
  3. Serge L. Beaucage
    Pages 33-61
  4. Brian C. Froehler
    Pages 63-80
  5. Masad J. Damha, Kelvin K Ogilvie
    Pages 81-114
  6. Brian S. Sproat
    Pages 115-141
  7. Richard I. Hogrefe, Mark A. Reynolds, Morteza M. Vaghefi, Kevin M. Young, Timothy A. Riley, Robert E. Klem et al.
    Pages 143-164
  8. Gerald Zon
    Pages 165-189
  9. W. T. Wiesler, W. S. Marshall, M. H. Caruthers
    Pages 191-206
  10. Maria Koziolkiewicz, Andrzej Wilk
    Pages 207-224
  11. Barbara Ramsay Shaw, Jon Madison, Anup Sood, Bernard F. Spielvogel
    Pages 225-243
  12. Wojciech Dabkowski, Jan Michahki, Friedrich Cramer
    Pages 245-260
  13. François Morvan, Bernard Rayner, Jean-Louis Imbach
    Pages 261-283
  14. Wojciech J. Stec, Zbigniew J. Lesnikowski
    Pages 285-313
  15. H. Seliger
    Pages 391-435
  16. Back Matter
    Pages 497-502

About this book

Introduction

When first conceived, not only was the aim of Protocols for Oligo­ nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe­ sizers has become a common practice in numerous laboratories. How­ ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle­ otide analogs have been synthesized and studied for their individual prop­ erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo­ nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.

Editors and affiliations

  • Sudhir Agrawal
    • 1
  1. 1.Worcester Foundation for Experimental BiologyShrewsbury

Bibliographic information

  • DOI https://doi.org/10.1385/0896032817
  • Copyright Information Humana Press 1993
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-281-1
  • Online ISBN 978-1-59259-507-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site