Table of contents

  1. Front Matter
    Pages i-xvii
  2. Protocols

    1. Front Matter
      Pages 1-1
    2. Nicoletta Archidiacono, Rachele Antonacci, Antonino Forabosco, Mariano Rocchi
      Pages 1-13
    3. William G. Kearns, Peter L. Pearson
      Pages 15-22
    4. Søren Pedersen, Johnny Hindkjær, Carsten A. Brandt, Lars Bolund, Steen Kølvraa
      Pages 23-33
    5. Henry H. Q. Heng, Lap-Chee Tsui
      Pages 35-49
    6. Christoph Lengauer, Ian Dunham, Terry Featherstone, Thomas Cremer
      Pages 51-61
    7. Christoph Lengauer, Michael R. Speicher, Thomas Cremer
      Pages 85-94
    8. Johnny Hindkjæer, Jørn Koch, Jens Mogensen, Steen Kølvraa, Lars Bolund
      Pages 95-107
    9. Henry H. Q. Heng, Lap-Chee Tsui
      Pages 109-122
    10. Barbara Spyropoulos, Peter B. Moens
      Pages 131-139
    11. Elizabeth Earle, K. H. Andy Choo
      Pages 147-158
    12. Pino Maccarone, Stephen A. Wilcox, Jaclyn M. Watson, Jennifer A. Marshall-Graves
      Pages 159-172
    13. Robert D. C. Saunders
      Pages 183-192

About this book


In situ hybridization is now recognized as an important technique in many areas of molecular biological research and its associated cli- cal studies. There is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense of urgency to acquire and extend the range of this technique in order not to miss out on the large body of information that can result from its application. During the explosive growth phase of most technical innovations, such knowledge is dif- cult to acquire systematically. The aim of In Situ Hybridization Pro- cols then is to bring together a wide range of detailed laboratory protocols covering different areas of the in situ hybridization technique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains an abundance of information and troubleshooting tips that come straight from the horse's mouth, and that are not often given in the restricted methods sections of standard research publications. For this reason, the book should also appeal to experienced workers wi- ing to add new methods to their repertoires.

Editors and affiliations

  • K. H. Andy Choo
    • 1
  1. 1.Murdoch InstituteMelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-280-4
  • Online ISBN 978-1-59259-520-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book