PCR Protocols

Current Methods and Applications

  • Bruce A. White

Part of the Methods in Molecular Biology book series (MIMB, volume 15)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White
    Pages 1-29
  3. Tim McDaniel, Stephen J. Meltzer
    Pages 41-43
  4. Udo Reischl, Rüdiger Rüger, Christoph Kessler
    Pages 51-62
  5. Shizhong Chen, Glen A. Evans
    Pages 75-80
  6. Matthias Volkenandt, Adam P. Dicker, Renato Fanin, Debabrata Banerjee, Anthony Albino, Joseph R. Bertino
    Pages 81-88
  7. Michael Jaye, Craig A. Dionne
    Pages 89-94
  8. Stephen C. Bain, John A. Todd
    Pages 95-112
  9. Adam P. Dicker, Matthias Volkenandt, Joseph R. Bertino
    Pages 143-152
  10. Gerd P. Pfeifer, Arthur D. Riggs
    Pages 153-168
  11. Alan R. Shuldiner, Riccardo Perfetti, Jesse Roth
    Pages 169-176
  12. Tetsuro Horikoshi, Kathleen Danenberg, Matthias Volkenandt, Thomas Stadlbauer, Peter V. Danenberg
    Pages 177-188
  13. Alice L. Witsell, Lawrence B. Schook
    Pages 199-204

About this book

Introduction

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

Editors and affiliations

  • Bruce A. White
    • 1
  1. 1.University of Connecticut Health CenterFarmington

Bibliographic information

  • DOI https://doi.org/10.1385/0896032442
  • Copyright Information Humana Press 1993
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-244-6
  • Online ISBN 978-1-59259-502-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book