New Nucleic Acid Techniques

  • John M. Walker
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 4)

Table of contents

  1. Front Matter
    Pages i-xvi
    PDF
  2. Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde
    Christopher F. Thurston, Caroline R. Perry, Jeffrey W. Pollard
    Pages 1-11
  3. Northern Blotting
    Jeffrey W. Pollard, Caroline R. Perry, Christopher F. Thurston
    Pages 13-25
  4. Hybrid-Release Translation
    Adrian Slater
    Pages 27-38
  5. Hybrid-Arrested Translation
    Keith Dudley
    Pages 39-45
  6. In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells
    Jeffrey W. Pollard, Michael J. Clemens
    Pages 47-60
  7. Use of Elutips to Purify DNA
    Paul D. Van Helden
    Pages 61-67
  8. Plasmid Preparation on Sephacryl S1000
    Paul D. Van Helden, Eileen G. Hoal
    Pages 69-74
  9. Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
    Robert McGookin
    Pages 75-79
  10. Use of 35S Nucleotides for DNA Sequencing
    Paul D. Van Helden
    Pages 81-88
  11. Direct Dideoxy DNA Sequencing
    Alexander Graham, J. Steven, D. McKechnie
    Pages 89-101
  12. Computer Applications to Molecular Biology
    Robert Harr, Petter Gustafsson
    Pages 103-138
  13. Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique
    Mark A. Plumb, Graham H. Goodwin
    Pages 139-164
  14. Oligonucleotide Synthesis Using the Manual Phosphotriester Method
    Daniel M. O’Callaghan, William J. Donnelly
    Pages 165-192
  15. Manual Oligonucleotide Synthesis Using the Phosphoramidite Method
    H. A. White
    Pages 193-213
  16. Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis
    Robert McGookin
    Pages 215-220
  17. Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries
    John Steven, Douglas McKechnie, Alexander Graham
    Pages 221-234
  18. Construction of Mammalian Genomic Libraries Using λ Replacement Vectors
    Alan N. Bateson, Jeffrey W. Pollard
    Pages 235-255
  19. Cosmid Library Construction
    John D. Haley
    Pages 257-283
  20. Construction of cDNA Libraries in λgt10 or λgt11
    Michael M. Burrell
    Pages 285-299

About this book

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.The Hatfield PolytechnicHatfieldUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896031276
  • Copyright Information Humana Press 1988
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-127-2
  • Online ISBN 978-1-59259-491-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book