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New Nucleic Acid Techniques

  • John M. Walker
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 4)

Table of contents

  1. Front Matter
    Pages i-xvi
    PDF
  2. Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde
    Christopher F. Thurston, Caroline R. Perry, Jeffrey W. Pollard
    Pages 1-11
  3. Northern Blotting
    Jeffrey W. Pollard, Caroline R. Perry, Christopher F. Thurston
    Pages 13-25
  4. Hybrid-Release Translation
    Adrian Slater
    Pages 27-38
  5. Hybrid-Arrested Translation
    Keith Dudley
    Pages 39-45
  6. In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells
    Jeffrey W. Pollard, Michael J. Clemens
    Pages 47-60
  7. Use of Elutips to Purify DNA
    Paul D. Van Helden
    Pages 61-67
  8. Plasmid Preparation on Sephacryl S1000
    Paul D. Van Helden, Eileen G. Hoal
    Pages 69-74
  9. Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
    Robert McGookin
    Pages 75-79
  10. Use of 35S Nucleotides for DNA Sequencing
    Paul D. Van Helden
    Pages 81-88
  11. Direct Dideoxy DNA Sequencing
    Alexander Graham, J. Steven, D. McKechnie
    Pages 89-101
  12. Computer Applications to Molecular Biology
    Robert Harr, Petter Gustafsson
    Pages 103-138
  13. Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique
    Mark A. Plumb, Graham H. Goodwin
    Pages 139-164
  14. Oligonucleotide Synthesis Using the Manual Phosphotriester Method
    Daniel M. O’Callaghan, William J. Donnelly
    Pages 165-192
  15. Manual Oligonucleotide Synthesis Using the Phosphoramidite Method
    H. A. White
    Pages 193-213
  16. Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis
    Robert McGookin
    Pages 215-220
  17. Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries
    John Steven, Douglas McKechnie, Alexander Graham
    Pages 221-234
  18. Construction of Mammalian Genomic Libraries Using λ Replacement Vectors
    Alan N. Bateson, Jeffrey W. Pollard
    Pages 235-255
  19. Cosmid Library Construction
    John D. Haley
    Pages 257-283
  20. Construction of cDNA Libraries in λgt10 or λgt11
    Michael M. Burrell
    Pages 285-299
  21. Immunoscreening of λgt11 Expression Libraries Using an Avidin–Biotin Detection System
    Robert McGookin
    Pages 301-305
  22. Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides
    John D. Haley
    Pages 307-318
  23. Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors
    Keith K. Stanley, Joachim Herz, Harald Haymerle
    Pages 319-328
  24. Expression Screening of cDNA Libraries in pEX
    Keith K. Stanley
    Pages 329-342
  25. Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins
    Keith K. Stanley
    Pages 343-349
  26. Epitope Mapping Using pEX
    Keith K. Stanley
    Pages 351-361
  27. DNA Transfection of Mammalian Cells Using Polybrene
    William G. Chaney, Daniel R. Howard, Jeffrey W. Pollard, Sandra Sallustio, Pamela Stanley
    Pages 363-370
  28. Expression of Foreign Genes in Mammalian Cells
    Martin J. Page
    Pages 371-384
  29. Dot-Blot Hybridization Method
    Hans E. W. Bergmans, Wim Gaastra
    Pages 385-390
  30. Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes
    Domingo Gallardo, Albert Boronat
    Pages 391-399
  31. Photobiotin-Labeled DNA and RNA Hybridization Probes
    James L. McInnes, Anthony C. Forster, Robert H. Symons
    Pages 401-414
  32. Enzyme-Labeled DNA Probes
    J. Lesley Woodhead, Rachel Fallon, Hermia Figueiredo, Alan D. B. Malcolm
    Pages 415-420
  33. Luminescent Detection of Specific DNA Sequences
    J. Lesley Woodhead, Hermia Figueiredo, Alan D. B. Malcolm
    Pages 421-424
  34. Detection of DNA Sequences Using Biotinylated Probes
    J. Lesley Woodhead, Hermina Figueiredo, Alan D. B. Malcolm
    Pages 425-430
  35. Crosslinking of Single-Stranded DNA to Resins
    J. Lesley Woodhead, Jane A. Langdale, Alan D. B. Malcolm
    Pages 431-436
  36. Extraction of RNA from Plants
    Adrian Slater
    Pages 437-446
  37. Isolation of Plant Nuclei
    Richard D. Henfrey, Robert J. Slater
    Pages 447-452
  38. In Vitro Transcription in Plant Nuclei
    Richard D. Henfrey, Robert J. Slater
    Pages 453-463
  39. Large-Scale Isolation of Ti Plasmid DNA
    Kevan M. A. Gartland
    Pages 465-470
  40. Transformation and Regeneration of Dicotyledonous Plants
    Kevan M. A. Gartland, Nigel W. Scott
    Pages 471-479
  41. Plant Protoplast Fusion
    Neil Fish, Keith Lindsey, Michael G. K. Jones
    Pages 481-498
  42. Plant Tissue Culture
    Michael G. K. Jones, Neil Fish, Keith Lindsey
    Pages 499-517
  43. Direct Gene Transfer into Plant Protoplasts
    Keith Lindsey, Michael G. K. Jones, Neil Fish
    Pages 519-536
  44. Isolation of Wheat Chloroplasts and Chloroplast DNA
    Matthew Clement Jones
    Pages 537-544
  45. DNA Labeling by Isolated Wheat Chloroplasts
    Matthew Clement Jones
    Pages 545-553
  46. Back Matter
    Pages 555-560
    PDF

About this book

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.The Hatfield PolytechnicHatfieldUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896031276
  • Copyright Information Humana Press 1988
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-127-2
  • Online ISBN 978-1-59259-491-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site