DNA Vaccines

Methods and Protocols

  • W. Mark Saltzman
  • Hong Shen
  • Janet L. Brandsma

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 127)

Table of contents

  1. Front Matter
    Pages i-xv
  2. DNA Vaccine Design

    1. Front Matter
      Pages 1-1
    2. Janet L. Brandsma
      Pages 3-10
    3. Donna L. Montgomery, Kristala Jones Prather
      Pages 11-22
    4. Steve Pascolo
      Pages 23-40
    5. Petra Riedl, Nicolas Fissolo, Jörg Reimann, Reinhold Schirmbeck
      Pages 41-53
    6. Weiwen Jiang, Charles F. Reich, David S. Pisetsky
      Pages 55-70
  3. DNA Vaccine Delivery Systems

    1. Front Matter
      Pages 71-71
    2. Shawn Babiuk, Sylvia van Drunen Littel-van den Hurk, Lorne A. Babiuk
      Pages 73-82
    3. Kanakatte Raviprakash, Kevin R. Porter
      Pages 83-89
    4. Sylvia van Drunen Littel-van den Hurk, Shawn Babiuk, Lorne A. Babiuk
      Pages 91-105
    5. Mark E. Keegan, W. Mark Saltzman
      Pages 107-113
  4. DNA Vaccine Adjuvants and Activity Enhancement

    1. Front Matter
      Pages 137-137
    2. C. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas et al.
      Pages 171-197
    3. Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang W. Leitner, Josef Thalhamer
      Pages 221-235
  5. DNA Vaccine Applications

    1. Front Matter
      Pages 237-237
    2. Martha Sedegah, Stephen L. Hoffman
      Pages 239-251
    3. Richard Weiss, Sandra Scheiblhofer, Josef Thalhamer
      Pages 253-267
  6. DNA Vaccine Production, Purification, and Quality

    1. Front Matter
      Pages 293-293
    2. Kristin Listner, Laura Kizer Bentley, Michel Chartrain
      Pages 295-309
    3. Joachim Schorr, Peter Moritz, Astrid Breul, Martin Scheef
      Pages 339-350
    4. Jason C. Murphy, Michael A. Winters, Sangeetha L. Sagar
      Pages 351-362
    5. James S. Robertson, Elwyn Griffiths
      Pages 363-374
  7. Back Matter
    Pages 375-384

About this book


In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.


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Editors and affiliations

  • W. Mark Saltzman
    • 1
  • Hong Shen
    • 2
  • Janet L. Brandsma
    • 3
  1. 1.Department of Biomedical EngineeringYale UniversityNew Haven
  2. 2.Department of Chemical EngineeringUniversity of WashingtonSeattle
  3. 3.Section of Comparative MedicineYale UniversityNew Haven

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-484-5
  • Online ISBN 978-1-59745-168-0
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site