Adenovirus Methods and Protocols

Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

  • William S. M. Wold
  • Ann E. Tollefson

Part of the Methods in Molecular Medicine book series (MIMM, volume 130)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Julie Boyer, Gary Ketner
    Pages 1-17
  3. Angela N. Cauthen, Amanda R. Welton, Katherine R. Spindler
    Pages 41-59
  4. P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti
    Pages 61-68
  5. Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw
    Pages 69-90
  6. Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo
    Pages 91-106
  7. Jason Gall, John Schoggins, Erik Falck-Pedersen
    Pages 107-123
  8. Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian, Prem Seth
    Pages 135-144
  9. Karoly Toth, Jacqueline F. Spencer, William S. M. Wold
    Pages 157-168
  10. Maria A. Thomas, Jacqueline F. Spencer, William S. M. Wold
    Pages 169-183
  11. Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, William S. M. Wold
    Pages 185-192
  12. Graham Bottley, John R. Holt, Nicola J. James, G. Eric Blair
    Pages 205-213
  13. Amanda R. Welton, Katherine R. Spindler
    Pages 215-221
  14. Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova, Konstantin Doronin, William S. M. Wold
    Pages 223-235
  15. Back Matter
    Pages 237-241

About this book


Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.


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Editors and affiliations

  • William S. M. Wold
    • 1
  • Ann E. Tollefson
    • 1
  1. 1.Department of Molecular Microbiology and ImmunologySaint Louis University School of MedicineSt. Louis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-598-9
  • Online ISBN 978-1-59745-166-6
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site