Dictyostelium discoideum Protocols

  • Ludwig Eichinger
  • Francisco Rivero

Part of the Methods in Molecular Biology™ book series (MIMB, volume 346)

Table of contents

  1. Front Matter
    Pages i-xv
  2. The Organism, Community Resources, and Genome-wide Studies

    1. Front Matter
      Pages 1-1
    2. Richard H. Kessin
      Pages 3-13
    3. Adam Kuspa, William F. Loomis
      Pages 15-29
    4. Hideko Urushihara, Takahiro Morio, Yoshimasa Tanaka
      Pages 31-49
    5. Petra Fey, Pascale Gaudet, Karen E. Pilcher, Jakob Franke, Rex L. Chisholm
      Pages 51-74
    6. Marcel Kaul, Ludwig Eichinger
      Pages 75-93
    7. Udo Roth, Stefan Müller, Franz-Georg Hanisch
      Pages 95-109
  3. Basic Methods and Molecular Genetic Techniques

    1. Front Matter
      Pages 111-111
    2. Hideko Urushihara
      Pages 113-124
    3. Jason King, Robert Insall
      Pages 125-135
    4. Paul R. Fisher, Sarah J. Annesley
      Pages 137-170
    5. Markus Kuhlmann, Blagovesta Popova, Wolfgang Nellen
      Pages 211-226
  4. Imaging and Localization Methods

    1. Front Matter
      Pages 227-227
    2. Ricardo Escalante, Leandro Sastre
      Pages 247-260
    3. David Woznica, David A. Knecht
      Pages 311-325
    4. Monica Hagedorn, Eva M. Neuhaus, Thierry Soldati
      Pages 327-338
    5. Mark J. Grimson, Richard L. Blanton
      Pages 339-365
  5. Dictyostelium as Model Organism

    1. Front Matter
      Pages 367-367
    2. Peter J. M. Van Haastert
      Pages 369-392
    3. Michelle C. Mendoza, Richard A. Firtel
      Pages 393-405
    4. Francisco Rivero, Markus Maniak
      Pages 423-438
    5. Daniel Gotthardt, Régis Dieckmann, Vincent Blancheteau, Claudia Kistler, Frank Reichardt, Thierry Soldati
      Pages 439-448
    6. Salvatore Bozzaro
      Pages 449-467
    7. Irene Schulz, Yvonne Reinders, Albert Sickmann, Ralph Gräf
      Pages 479-489
    8. Markus Kaller, Wolfgang Nellen, Jonathan R. Chubb
      Pages 491-505
    9. Kathryn E. Adley, Melanie Keim, Robin S. B. Williams
      Pages 517-534
    10. Artemis Kosta, Catherine Laporte, David Lam, Emilie Tresse, Marie-Françoise Luciani, Pierre Golstein
      Pages 535-550
  6. Back Matter
    Pages 551-564

About this book


Dictyostelium discoideum Protocols presents a comprehensive collection of cutting-edge molecular biological protocols for studying D. discoideum, with emphasis on its utility in the study of fundamental cellular processes including signal transduction, chemotaxis, cell motility, cytokinesis, phagocytosis, and aspects of development such as cell sorting, pattern formation, and cell type differentiation. Written by experts in the field, this important guide provides easy access to current methods and techniques for the study of D. discoideum. For the newcomer to the field, it offers a complete and inclusive introduction to this important model organism. The book is divided into four main parts, which include an introduction to the organism that provides integral community resources and genome-wide approaches; basic methods and available molecular genetic techniques for study of the organism; imaging and localization methods; and a discussion of D. discoideum, with emphasis on its unique advantages as a model system.
This in-depth guide provides a complete introduction to and thorough handbook for the study of D. discoideum, including a discussion of the implications of the recently completed Dictyostelium genome project in strengthening the position of D. discoideum as a model organism for studying fundamental cell processes and aspects of development. Researchers at all levels of experience will benefit from the presentations throughout of the most current, useful, and innovative techniques for the study of D. discoideum from leading Dictyostelium scientists.


Activation DNA sequencing Endoplasmatisches Reticulum Microarray Vivo cellular processes gene expression genes hybridization signal transduction

Editors and affiliations

  • Ludwig Eichinger
    • 1
  • Francisco Rivero
    • 1
  1. 1.Center for Biochemistry and Center for Molecular Medicine CologneUniversity of CologneCologneGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-623-8
  • Online ISBN 978-1-59745-144-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site