Fluorescent Energy Transfer Nucleic Acid Probes

Designs and Protocols

  • Vladimir V. Didenko

Part of the Methods in Molecular Biology™ book series (MIMB, volume 335)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Design of Energy Transfer Probes

  3. Energy Transfer Probes for DNA and RNA Hybridization Detection and Monitoring

  4. Energy Transfer Probes for DNA Breaks Detection and DNA Cleavage Monitoring

    1. Front Matter
      Pages 57-57
    2. John B. Biggins, James R. Prudent, David J. Marshall, Jon S. Thorson
      Pages 83-92
  5. Monitoring of DNA Synthesis and Amplification Using Energy Transfer Probes

  6. DNA Sequence Analysis and Mutation Detection Using Fluorescence Energy Transfer

About this book

Introduction

Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have multiplied in recent years, until now there have been no manuals summarizing the many different protocols and probe designs. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is the first publication to provide such a collection. In the volume, hands-on experts-often the original developers of the technique-comprehensively describe a variety of fluorescent probes and devices that use ET, including molecular beacons, molecular break lights, TaqMan® probes, LUX™ primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and logic gates for molecular-scale computation. Merging work on nanotechnology and fluorescent probes, the authors assemble the first comprehensive treatment of all the key DNA- and RNA-based ET probes and present strategies for their optimized custom design. They discuss both fluorescence resonance energy transfer (FRET)-based and non-FRET-based constructs, and provide a complete set of techniques to monitor DNA and RNA reactions, such as hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Detailed protocols are provided for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, and single-nucleotide polymorphisms. The protocols follow the successful Methods in Molecular Biology™ series format, each offering an outline of the principles behind the technique, step-by-step detailed instructions, and comprehensive troubleshooting.
Exhaustive and state-of-the-art, Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is an invaluable resource for both novice and experienced researchers who wish to use the latest developments in fluorescent probes in any field of molecular biology.

Keywords

DNA Genotyp Mutation Single Nucleotide Polymorphism Termination gene expression genes hybridization

Editors and affiliations

  • Vladimir V. Didenko
    • 1
  1. 1.Baylor College of MedicineHouston

Bibliographic information

  • DOI https://doi.org/10.1385/1597450693
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-380-0
  • Online ISBN 978-1-59745-069-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book