Human Embryonic Stem Cell Protocols

  • Kursad Turksen

Part of the Methods In Molecular Biology book series (MIMB, volume 331)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Kursad Turksen, Tammy-Claire Troy
    Pages 1-12
  3. Mark Richards, Ariff Bongso
    Pages 23-41
  4. Michal Amit, Joseph Itskovitz-Eldor
    Pages 43-53
  5. Maisam Mitalipova, Gail Palmarini
    Pages 55-76
  6. Jim McWhir, Davina Wojtacha, Alison Thomson
    Pages 77-90
  7. Gautam Dravid, Holly Hammond, Linzhao Cheng
    Pages 91-104
  8. Melissa Carpenter, Mahendra S. Rao, William J. Freed, Xianmin Zeng
    Pages 153-167
  9. Lisheng Wang, Chantal Cerdan, Pablo Menendez, Mickie Bhatia
    Pages 179-200
  10. Pablo Menendez, Lisheng Wang, Chantal Cerdan, Mickie Bhatia
    Pages 201-220
  11. Yongquan Luo, Bhaskar Bhattacharya, Amy X. Yang, Raj K. Puri, Mahendra S. Rao
    Pages 241-266
  12. Daixing Zhou, Mahendra S. Rao, Roger Walker, Irina Khrebtukova, Christian D. Haudenschild, Takumi Miura et al.
    Pages 285-311
  13. Maria Wartenberg, Andreas Finkensieper, Jürgen Hescheler, Heinrich Sauer
    Pages 313-328
  14. Justin C. St. John, Alexandra Amaral, Emma Bowles, João Facucho Oliveira, Rhiannon Lloyd, Mariana Freitas et al.
    Pages 347-374
  15. Back Matter
    Pages 375-385

About this book


Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book.
Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.


DNA biology cancer cell microarray mitochondria molecular biology stem cell stem cells tissue

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press Inc. 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-497-5
  • Online ISBN 978-1-59745-046-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site