© 2006

Embryonic Stem Cell Protocols

Volume 1: Isolation and Characterization

  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 329)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Isolation and Maintenance

    1. Front Matter
      Pages 1-1
    2. Yunhan Hong, Manfred Schartl
      Pages 3-16
    3. Hiroyuki Horiuchi, Shuichi Furusawa, Haruo Matsuda
      Pages 17-34
    4. Maren Schulze, Hendrik Ungefroren, Michael Bader, Fred Fändrich
      Pages 45-58
    5. Shigeo Saito, Ken Sawai, Arika Minamihashi, Hideyo Ugai, Takehide Murata, Kazunari K. Yokoyama
      Pages 59-79
    6. Hirofumi Suemori, Norio Nakatsuji
      Pages 81-89
  3. Signaling in Embryonic Stem Cell Differentiation

    1. Front Matter
      Pages 99-99
    2. Shaohua Li, Peter D. Yurchenco
      Pages 113-125
    3. Gabriella Minchiotti, Silvia Parisi, M. Graziella Persico
      Pages 151-170
    4. Sandy Becker, Laura Grabel
      Pages 171-185
    5. Sangmi Chung, Kwang-Soo Kim
      Pages 187-193
    6. Kenneth R. Boheler, Kirill V. Tarasov
      Pages 195-221
    7. Yoshimi Tokuzawa, Masayoshi Maruyama, Shinya Yamanaka
      Pages 223-231
    8. J. Matthew Velkey, Nicole A. Slawny, Theresa E. Gratsch, K. Sue O’shea
      Pages 233-261
  4. Genetic Manipulation of Embryonic Stem Cells

    1. Front Matter
      Pages 263-263

About this book


Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.


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Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • Book Title Embryonic Stem Cell Protocols
  • Book Subtitle Volume 1: Isolation and Characterization
  • Editors Kursad Turksen
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-498-2
  • Softcover ISBN 978-1-61737-601-6
  • eBook ISBN 978-1-59745-037-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XXI, 510
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site


From Reviews of the First Edition...

"...elegantly introduces tremendous methods and protocols in ES of the most useful books that I have ever read in this field..." -Cell Biology International

"...highly valuable for any scientist who wants to make a start in the exciting field but also for experienced ES cell researchers who want to widen their repertoire" -Diabetologia

"...a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research" -Molecular Biotechnology

"...a useful companion volume to other more specialized ES cell books..." -Nature Cell Biology

From the reviews of the second edition:

"Volume 1 describes methods for stem cell isolation and characterization. … written for basic science and clinical researchers interested in cellular and developmental biology, biochemistry, and molecular biology. Biomedical researchers interested in bioengineering, gene therapy, oncology, and regenerative medicine will also appreciate the methods described here. … The protocols are typically organized around specific research questions, and numerous examples of research findings are presented. … a welcome resource for research scientists interested in stem cell biology." (Bruce A Fenderson, Doody’s Review Service, July, 2006)