Embryonic Stem Cell Protocols

Volume 2: Differentiation Models

  • Kursad Turksen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 330)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Isolation and Maintenance

  3. Signaling in Embryonic Stem Cell Differentiation

    1. Renée F. de Pooter, Thomas M. Schmitt, Juan Carlos Zúñiga-Pflücker
      Pages 113-121
    2. Rebecca J. Chan, Robert Hromas, Mervin C. Yoder
      Pages 123-133
    3. Nicole L. Woll, Sarah K. Bronson
      Pages 149-159
    4. Jaspal Singh Khillan
      Pages 161-170
    5. Jan Kramer, Gunnar Hargus, Jürgen Rohwedel
      Pages 171-190
    6. Cornelia Gissel, Michael Xavier Doss, Rita Hippler-Altenburg, Jürgen Hescheler, Agapios Sachinidis
      Pages 191-219
    7. Lijuan L. Shang, Samuel C. Dudley Jr., Arnold E. Pfahnl
      Pages 221-231
  4. Genetic Manipulation of Embryonic Stem Cells

    1. Kara E. McCloskey, Steven L. Stice, Robert M. Nerem
      Pages 287-301
    2. Clotilde Gimond, Sandrine Marchetti, Gilles Pagés
      Pages 303-329
    3. Tetsuro Watabe, Jun K. Yamashita, Koichi Mishima, Kohei Miyazono
      Pages 341-351
  5. Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens

    1. Johan Saldeen, Nina Ågren, Björn Åkerblom, Lingge Lu, Bashir Adem, Łukasz Sędek et al.
      Pages 353-372
    2. Przemyslaw Blyszczuk, Anna M. Wobus
      Pages 373-385
    3. Shigeaki Ishizaka, Yukiteru Ouji, Masahide Yoshikawa, Kazuki Nakatani
      Pages 387-399
    4. Xing Zhao, Jianuo Liu, Iqbal Ahmad
      Pages 401-416
  6. Epigenetic Analysis of Embryonic Stem Cells

  7. Back Matter
    Pages 445-456

About this book


Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. A companion first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, focuses on ES cells recently isolated from different nonhuman species, to provide a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.


Vivo biology cell cell culture cell differentiation development neural stem cells protein stem cell stem cells tissue

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-784-6
  • Online ISBN 978-1-59745-036-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site