Arabidopsis Protocols

  • Julio Salinas
  • Jose J. Sanchez-Serrano

Part of the Methods in Molecular Biology™ book series (MIMB, volume 323)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Growing Arabidopsis

    1. Front Matter
      Pages 1-1
    2. Luz Rivero-Lepinckas, Deborah Crist, Randy Scholl
      Pages 3-12
    3. Jenifer Bush, Georg Jander, Frederick M. Ausubel
      Pages 13-25
    4. Krithika Yogeeswaran, Mikhail Elia Nasrallah
      Pages 27-38
    5. Katherine Bainbridge, Tom Bennett, Colin Turnbull, Ottoline Leyser
      Pages 39-44
  3. Genetic Analyses

    1. Front Matter
      Pages 63-63
    2. Maarten Koornneef, Carlos Alonso-Blanco, Piet Stam
      Pages 65-77
    3. Carlos Alonso-Blanco, Maarten Koornneef, Johan W. van Ooijen
      Pages 79-99
    4. YongSig Kim, Karen S. Schumaker, Jian-Kang Zhu
      Pages 101-103
    5. M. Rosa Ponce, Pedro Robles, Francisca M. Lozano, M. Asunción Brotóns, José L. Micol
      Pages 105-113
    6. Bradley J. Till, Trenton Colbert, Christine Codomo, Linda Enns, Jessica Johnson, Steven H. Reynolds et al.
      Pages 127-135
    7. Anja Schneider, Dario Leister
      Pages 147-161
    8. Anna N. Stepanova, Jose M. Alonso
      Pages 163-172
    9. Martin Lysak, Paul Fransz, Ingo Schubert
      Pages 173-186
    10. Margarita Garcia-Hernández, Leonore Reiser
      Pages 187-211
  4. Transient and Stable Transformation

    1. Front Matter
      Pages 213-213

About this book

Introduction

Emulating the much-praised first edition of Arabidopsis Protocols, leading scientists have generated an up-to-date work that reflects recent advances in plant biology, the completion of the Arabidopsis genome sequence-essential for studying plant function-and the development of whole systems approaches that allow global analysis of gene expression, as well as protein and metabolite dynamics. The authors have included nearly all techniques developed in Arabidopsis, others recently adapted from more traditional work in crop species, and the latest using Arabidopsis as a model system. Highlights include the most recent methods-transcriptomics, proteomics, and metabolomics-and their novel applications (phosphoproteomics, DNA microarray-based genotyping, high throughput metabolite profiling, and single-cell RNA). Traditional protocols from the agricultural sciences and others developed in crop species (grafting and chloroplast transformation) have been adapted to exploit the advantages of the Arabidopsis model. The protocols themselves follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Indispensable and highly practical, Arabidopsis Protocols, Second Edition offers both novice and experienced plant biologists cutting-edge tools to explore new scenarios and gain an understanding of how this complex, multicellular organism works, how it copes with a sessile life style, and how these strategies compare with those developed in other organisms.

Keywords

DNA Embryo Genotyp Mutant Mutation PCR Seed arabidopsis thaliana biotechnology gene expression genes genetic engineering hybridization transcription

Editors and affiliations

  • Julio Salinas
    • 1
  • Jose J. Sanchez-Serrano
    • 2
  1. 1.Dept. of BiotechnologyInstituto Nacional de Investigacion y Tecnología Agraria y Alimentaria (INIA)MadridSpain
  2. 2.Dept. de Genetica Molecular de PlantasCentro Nacional de Biotecnología-CSIC, Campus de CantoblancoMadridSpain

Bibliographic information

  • DOI https://doi.org/10.1385/1597450030
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-395-4
  • Online ISBN 978-1-59745-003-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book