Hormone Assays in Biological Fluids

  • Michael J. Wheeler
  • J. S. Morley Hutchinson

Part of the Methods in Molecular Biology book series (MIMB, volume 324)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Michael J. Wheeler
    Pages 1-23
  3. John W. Honour
    Pages 53-74
  4. Gill Rumsby
    Pages 75-89
  5. Christine R. Squire
    Pages 91-108
  6. Michael J. Wheeler
    Pages 109-124
  7. Norman F. Taylor
    Pages 159-175
  8. Marion L. Cawood
    Pages 177-185
  9. Marion L. Cawood
    Pages 187-196
  10. Michael J. Wheeler
    Pages 197-211
  11. Shahrad Taheri, Mohammad Ghatei, Steve Bloom
    Pages 213-233
  12. Back Matter
    Pages 255-258

About this book

Introduction

Although automated systems can measure all common hormones in human blood, they are expensive and often incapable of measuring hormones in urine, saliva, and animals. In Hormone Assays in Biological Fluids, expert researchers who have developed and applied significant new assays describe in step-by-step detail readily reproducible methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can develop novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and polymerase chain reaction techniques help readers choose the best methods for their purpose. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Hormone Assays in Biological Fluids illuminates the many different methods available for a broad range of hormone assays, enabling researchers to choose the optimal existing methods or develop novel assays for their specialized purposes.

Keywords

Chromatography Mass Spectrometry PCR

Editors and affiliations

  • Michael J. Wheeler
    • 1
  • J. S. Morley Hutchinson
    • 2
  1. 1.St. Thomas’ HospitalGuy’s and St. Thomas’ NHS Foundation TrustLondonUK
  2. 2.University of AberdeenAberdeenUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592599869
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-005-2
  • Online ISBN 978-1-59259-986-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book