Breast Cancer Research Protocols

  • Susan A. Brooks
  • Adrian Harris

Part of the Methods in Molecular Medicine book series (MIMM, volume 120)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Preparation of Cell and Tissue Samples

    1. Front Matter
      Pages 1-1
    2. Linda Snell, Peter H. Watson
      Pages 3-24
    3. Russell Leek
      Pages 25-28
    4. Irene Boeddinghaus, Stephen R. D. Johnson
      Pages 29-41
    5. Robert J. Springall, ChlllLeryl E. Gillett
      Pages 43-50
    6. Naomi Robertson, Russell Leek
      Pages 55-59
    7. Naomi Robertson, Russell Leek
      Pages 61-63
    8. Ulrich Lehmann, Hans Kreipe
      Pages 65-75
  3. Markers of Clinical Outcome and Prognosis

    1. Front Matter
      Pages 77-77
    2. Celia Chao, Kelly M. McMasters
      Pages 91-111
    3. Michael Z. Gilcrease, Aysegul Sahin
      Pages 113-125
    4. Bharat Jasani, Anthony Douglas-Jones, Anthony Rhodes, Susan Wozniak, Peter J. Barrett-Lee, Julie Gee et al.
      Pages 127-146
    5. Fazlul H. Sarkar, Yiwei Li
      Pages 147-160
    6. Stephen B. Fox
      Pages 161-187
  4. Analysis of Tumor-Derived Proteins and Antigens

    1. Front Matter
      Pages 189-189
    2. Cheryl E. Gillett
      Pages 191-200
    3. Miriam V. Dwek, Sarah L. Rawlings
      Pages 231-243
    4. Manfred Schmitt, Alexandra S. Sturmheit, Anita Welk, Christel Schnelldorfer, Nadia Harbeck
      Pages 245-265
  5. Analysis of Genes and Gene Expression in Tumor Specimens

    1. Front Matter
      Pages 267-267
    2. Patricia Gorman, Rebecca Roylance
      Pages 269-295
    3. Jan K. Blancato, Mary Steele Williams, Robert B. Dickson
      Pages 297-307
    4. Rosemary Jeffery, Toby Hunt, Richard Poulsom
      Pages 323-346
    5. Bruce R. Westley, Felicity E. B. May
      Pages 363-388
    6. Barbro Linderholm, Torbjörn Norberg, Jonas Bergh
      Pages 389-401
    7. Chris Jones, Peter Simpson, Alan Mackay, Sunil R. Lakhani
      Pages 403-414
    8. Peter Simpson, Chris Jones, Alan Mackay, Sunil R. Lakhani
      Pages 415-424
  6. Studying Cancer Cell Behavior in Vitro and In Vivo

    1. Front Matter
      Pages 425-425
    2. Ute Reuning, Manfred Schmitt, Birgit Luber, Veronika Beck, Viktor Magdolen
      Pages 427-440
    3. Kum Kum Khanna, Magtouf Gatei, Gordon Tribbick
      Pages 441-452
    4. Jennifer MacGregor Schafer, V. Craig Jordan
      Pages 453-464
    5. Stephen D. Robinson, Stephen Wilson, Kairbaan M. Hodivala-Dilke
      Pages 465-477
    6. Ursula Valentiner, Susan A. Brooks, Udo Schumacher
      Pages 479-488
    7. Juliette Murray, William R. Miller, J. Michael Dixon
      Pages 489-502
    8. Louise E. Reynolds, Kairbaan M. Hodivala-Dilke
      Pages 503-509
  7. Back Matter
    Pages 511-517

About this book


Many new technologies involving large-scale analysis of gene expression, novel treatments, and emergent combined therapy approaches have opened fresh possibilities for understanding and effectively managing breast cancer. In Breast Cancer Research Protocols, active researchers and clinicians describe in detail both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Breast Cancer Research Protocols offers laboratory and clinical researchers the tools needed to illuminate breast cancer biology and advance the efficacy of its treatment today.


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Editors and affiliations

  • Susan A. Brooks
    • 1
  • Adrian Harris
    • 2
  1. 1.School of Biological and Molecular SciencesOxford Brookes UniversityHeadingtonUK
  2. 2.Medical Oncology UnitThe Churchill HospitalHeadingtonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-191-2
  • Online ISBN 978-1-59259-969-1
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site