Mast Cells

Methods and Protocols

  • Guha Krishnaswamy
  • David S. Chi

Part of the Methods in Molecular Biology book series (MIMB, volume 315)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Overview of Mast Cell Biology

    1. Front Matter
      Pages 1-1
    2. Guha Krishnaswamy, Omar Ajitawi, David S. Chi
      Pages 13-34
    3. Gregory D. Gregory, Melissa A. Brown
      Pages 35-50
  3. Identification of Mast Cells in Culture and in Tissue

    1. Front Matter
      Pages 51-51
    2. Lawrence B. Schwartz
      Pages 53-62
    3. Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, George A. Youngberg
      Pages 63-76
    4. Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr et al.
      Pages 77-90
    5. Nikolaos G. Frangogiannis, Mark L. Entman
      Pages 91-101
  4. Development of Mast Cells In Vitro

    1. Front Matter
      Pages 103-103
    2. Gernot Sellge, Stephan C. Bischoff
      Pages 123-138
  5. Mast Cell Signaling and Gene Expression

    1. Front Matter
      Pages 139-139
    2. Chuanfu Li, Jim Kelley, Tuanzhu Ha
      Pages 141-150
    3. Stephen C. Armstrong
      Pages 151-163
    4. Tsung-Hsien Tsai, Denise M. Milhorn, Shau-Ku Huang
      Pages 165-174
    5. Yuko Kawakami, Jiro Kitaura, Toshiaki Kawakami
      Pages 175-189
  6. Mast Cell Expression and Release of Inflammatory Mediators of Inflammatory Mediators

    1. Front Matter
      Pages 191-191
    2. David A. Johnson
      Pages 193-202
    3. David S. Chi, S. Matthew Fitzgerald, Guha Krishnaswamy
      Pages 203-215
    4. Kevin F. Breuel, W. Keith De Ponti
      Pages 217-230
    5. Jacqueline M. Langdon, Susan M. MacDonald
      Pages 231-243
    6. William L. Stone, Hongsong Yang, Min Qui
      Pages 245-256
  7. Mast Cell Interactions With Other Cell Types

    1. Front Matter
      Pages 273-273
    2. Kottarappat N. Dileepan, Daniel J. Stechschulte
      Pages 275-294
    3. Ido Bachelet, Ariel Munitz, Francesca Levi-Schaffer
      Pages 295-317
    4. Jérôme Pène, Florence Guilhot, Isabelle Cognet, Paul Guglielmi, Angélique Guay-Giroux, Jean-Yves Bonnefoy et al.
      Pages 319-329
  8. Novel Aspects of Mast Cell Activation and Regulation

    1. Front Matter
      Pages 331-331
    2. Deling Yin, Charles A. Stuart
      Pages 333-339
    3. Jim Kelley, Gregory Hemontolor, Walid Younis, Chuanfu Li, Guha Krishnaswamy, David S. Chi
      Pages 341-348
    4. Ann L. Baldwin
      Pages 349-360
  9. Roles of Mast Cells in Host Defense

    1. Front Matter
      Pages 361-361
    2. Christopher P. Shelburne, James B. McLachlan, Soman N. Abraham
      Pages 363-381
    3. David S. Chi, Elaine S. Walker, Fred E. Hossler, Guha Krishnaswamy
      Pages 383-392
    4. Michael Stassen, Angela Valeva, Iwan Walev, Edgar Schmitt
      Pages 393-403
  10. Analysis of Programmed Cell Death in Mast Cells

    1. Front Matter
      Pages 405-405
    2. Alexander Gerbaulet, Karin Hartmann, Yoseph A. Mekori
      Pages 407-423
  11. Back Matter
    Pages 425-439

About this book


The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.


Antigen DNA RNA biology cell biology cytokine development gene expression genes microarray molecular biology protein regulation reproducible techniques tissue

Editors and affiliations

  • Guha Krishnaswamy
    • 1
  • David S. Chi
    • 1
  1. 1.Quillen College of Medicine, James H. Quillen Veterans Affairs Medical CenterEast Tennessee State UniversityJohnson City

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-374-9
  • Online ISBN 978-1-59259-967-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site