Yeast Protocol

  • Wei Xiao

Part of the Methods in Molecular Biology book series (MIMB, volume 313)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Brendan P. G. Curran, Virginia Bugeja
    Pages 1-13
  3. Michelle Hanna, Wei Xiao
    Pages 15-20
  4. Ben Distel, Astrid Kragt
    Pages 21-26
  5. Barry Panaretou, Peter Piper
    Pages 27-32
  6. Chris Meisinger, Nikolaus Pfanner, Kaye N. Truscott
    Pages 33-39
  7. Roger Schneiter, Günther Daum
    Pages 41-45
  8. Hélian Boucherie, Christelle Monribot-Espagne
    Pages 47-64
  9. Laura Maringele, David Lydall
    Pages 65-73
  10. Roger Schneiter, Günther Daum
    Pages 75-84
  11. Jiří Hašek
    Pages 85-96
  12. Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, Sean M. Hemmingsen
    Pages 97-105
  13. R. Daniel Gietz, Robin A. Woods
    Pages 107-120
  14. Leslie Barbour, Michelle Hanna, Wei Xiao
    Pages 121-127
  15. Johannes H. Hegemann, Ulrich Güldener, Gabriele J. Köhler
    Pages 129-144
  16. Leslie Barbour, Wei Xiao
    Pages 161-169
  17. Amy Hin Yan Tong, Charles Boone
    Pages 171-191
  18. Alain T. Dandjinou, Michel Larrivée, Ralf E. Wellinger, Raymund J. Wellinger
    Pages 193-208
  19. Karen M. Robinson, Michael C. Schultz
    Pages 209-223

About this book

Introduction

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms.

Keywords

DNA Mutant Organelle gene expression genes saccharomyces cerevisiae transcription

Editors and affiliations

  • Wei Xiao
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of SaskatchewanSaskatoon, SaskatchewanCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592599583
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-437-1
  • Online ISBN 978-1-59259-958-5
  • Series Print ISSN 1064-3745
  • About this book