Fibrosis Research

Methods and Protocols

  • John Varga
  • David A. Brenner
  • Sem H. Phan

Part of the Methods in Molecular Medicine book series (MIMM, volume 117)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. The Clinical Scope of Fibrosis

    1. Front Matter
      Pages 1-1
    2. David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, Joseph P. Lynch III
      Pages 3-44
    3. H. William Schnaper
      Pages 45-68
    4. Alain Mauviel
      Pages 69-80
  3. Regulation of Extracellular Matrix Metabolism: Cultured Cells

    1. Front Matter
      Pages 81-81
    2. Laure Rittié, Gary J. Fisher
      Pages 83-98
    3. Ralf Weiskirchen, Axel M. Gressner
      Pages 99-113
    4. Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith et al.
      Pages 115-127
    5. David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, Ronald H. Goldstein
      Pages 129-140
    6. Richard A. Rippe, Branko Stefanovic
      Pages 141-160
    7. Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, Daniel B. Rifkin
      Pages 161-175
  4. Studying Fibrosis Using Animal Models

    1. Front Matter
      Pages 177-177
    2. Rakesh K. Kumar
      Pages 179-188
    3. Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, Steven L. Kunkel
      Pages 209-221
    4. Mary Birch, Annette Tomlinson, Mark W. J. Ferguson
      Pages 223-235
    5. Christothea Constandinou, Neil Henderson, John P. Iredale
      Pages 237-250
    6. Mehrnaz Gharaee-Kermani, Matthew Ullenbruch, Sem H. Phan
      Pages 251-259
    7. Michael Zeisberg, Mary A. Soubasakos, Raghu Kalluri
      Pages 261-272
    8. Yao Sun, Karl T. Weber
      Pages 273-290
  5. Genetic Approaches to Studying Fibrosis

    1. Front Matter
      Pages 291-291
    2. Lauranell H. Burch, David A. Schwartz
      Pages 293-313
    3. Nilesh B. Davé, Naftali Kaminski
      Pages 333-358
    4. Bernd Schnabl, Youkyung Hwang Choi, Curt H. Hagedorn, Ramón Bataller
      Pages 359-369
  6. Back Matter
    Pages 381-391

About this book


Fibrosis, or scar tissue, is increasingly recognized as an important feature of many chronic diseases of the lung, kidney, heart, skin, eye, and bone. With some 45% of deaths in the United States attributed to fibrosing disorders and the lack of any effective treatment, research into the cellular, molecular, and genetic basis of fibrosis has exploded. In Fibrosis Research: Methods and Protocols, leading investigators at the forefront of this emerging field review the highlights of current fibrosis work in a variety of disciplines and the experimental methodologies used to uncover the mechanisms that drive it. In their discussion of research techniques utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. Additional techniques presented cover the evolving genetic methods for identifying "fibrosis genes" or allelic polymorphisms in human populations, microarray studies for describing global patterns of gene expression associated with fibrosis, and proteomic approaches to the same. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and state-of-the-art, Fibrosis Research: Methods and Protocols offers investigators all the powerful experimental methodologies, techniques, and strategies needed today to study and ultimately understand the daunting problem of pathological fibrosis.


biopsy molecular medicine pathogenesis skin

Editors and affiliations

  • John Varga
    • 1
  • David A. Brenner
    • 2
  • Sem H. Phan
    • 3
  1. 1.Feinberg School of MedicineNorthwestern UniversityChicago
  2. 2.College of Physicians and SurgeonsColumbia UniversityNew York
  3. 3.University of Michigan Medical SchoolAnn Arbor

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-479-1
  • Online ISBN 978-1-59259-940-0
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site