Methods and Protocols

  • Tim Illidge
  • Peter W. M. Johnson

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 115)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Kim Last, Silvana Debarnardi, T. Andrew Lister
    Pages 15-63
  3. Andrew Jack, Sharon Barrans, David Blythe, Andrew Rawstron
    Pages 65-91
  4. Fiona M. Ross, Christine J. Harrison
    Pages 93-107
  5. Amanda P. Liggins, Barbara A. Guinn, Alison H. Banham
    Pages 109-128
  6. Surinder S. Sahota, Gavin Babbage, Niklas Zojer, Christian H. Ottensmeier, Freda K. Stevenson
    Pages 129-144
  7. Katy McCann, Surinder S. Sahota, Freda K. Stevenson, Christian H.
    Pages 145-171
  8. Elizabeth Hodges, Anthony P. Williams, Susan Harris, John L. Smith
    Pages 197-215
  9. E. Loraine Karran, Takashi Sonoki, Martin J. S. Dyer
    Pages 217-230
  10. Rachel E. Ibbotson, Anton E. Parker, David G. Oscier
    Pages 241-250
  11. Simon D. Wagner, Jaspal S. Kaeda
    Pages 251-269
  12. Karen Pulford, Helen M. Roberton, Margaret Jones
    Pages 271-294
  13. Angela Darby, John G. Gribben
    Pages 315-331
  14. Back Matter
    Pages 333-342

About this book


The tremendous progress made over the last few years in our molecular understanding of lymphocyte biology and lymphoma classification promises to have a major impact on clinical practice. In Lymphoma: Methods and Protocols, leading clinical investigators from world renowned laboratories describe in step-by-step detail the latest molecular techniques being used to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Lymphoma: Methods and Protocols offers oncologists, hematologists, and clinical scientists a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas.


DNA biopsy chemistry leukemia lymphoma

Editors and affiliations

  • Tim Illidge
    • 1
  • Peter W. M. Johnson
    • 2
  1. 1.CRUK, Paterson Institute for Cancer ResearchUniversity of ManchesterManchesterUK
  2. 2.Cancer Sciences DivisionSouthampton General HospitalSouthamptonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-159-2
  • Online ISBN 978-1-59259-936-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site