Therapeutic Proteins

Methods and Protocols

  • C. Mark Smales
  • David C. James

Part of the Methods in Molecular Biology™ book series (MIMB, volume 308)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. John R. Birch, Yemi Onakunle
    Pages 1-16
  3. Andrew G. Popplewell, Mukesh Sehdev, Mariangela Spitali, A. Neil C. Weir
    Pages 17-30
  4. Sang Yup Lee, Jong Hyun Choi, Sang Jun Lee
    Pages 31-41
  5. Lee J. Byrne, Kenneth J. O’Callaghan, Mick F. Tuite
    Pages 51-64
  6. Eric C. de Bruin, Erwin H. Duitman, Arjo L. de Boer, Marten Veenhuis, Ineke G. A. Bos, C. Erik Hack
    Pages 65-76
  7. Sabine Geisse, Martin Jordan, Florian M. Wurm
    Pages 87-98
  8. Bhaskar Thyagarajan, Michele P. Calos
    Pages 99-106
  9. Lily Chu, Ilse Blumentals, Gargi Maheshwari
    Pages 107-121
  10. Beat P. Kramer, Martin Fussenegger
    Pages 123-143
  11. Teruhisa Nakashima, Kazuhiko Tomokiyo
    Pages 177-190
  12. Alberto L. Horenstein, Ilaria Durelli, Fabio Malavasi
    Pages 191-208
  13. Nicola Boschetti, Anna Johnston
    Pages 209-219
  14. Marina Korneyeva, Scott Rosenthal
    Pages 221-231

About this book

Introduction

With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation.

Keywords

Antigen Filtration gene expression interferon proteins

Editors and affiliations

  • C. Mark Smales
    • 1
  • David C. James
    • 2
  1. 1.Protein Science Group, Department of BiosciencesUniversity of KentCanterburyUK
  2. 2.School of EngineeringUniversity of QueenslandSt. LuciaAustralia

Bibliographic information

  • DOI https://doi.org/10.1385/1592599222
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-390-9
  • Online ISBN 978-1-59259-922-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book