Human Retrovirus Protocols

Virology and Molecular Biology

  • Tuofu Zhu

Part of the Methods in Molecular Biology™ book series (MIMB, volume 304)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Methods for Virus Isolation and Detection

    1. Front Matter
      Pages 2-2
    2. Paul R. Gorry, Secondo Sonza, Katherine Kedzierska, Suzanne M. Crowe
      Pages 25-33
    3. Kathleen Borgmann, Howard E. Gendelman, Anuja Ghorpade
      Pages 49-70
    4. Joëlle Tobaly-Tapiero, Patricia Bittoun, Ali Saïb
      Pages 125-137
    5. Audrey Brussel, Olivier Delelis, Pierre Sonigo
      Pages 139-154
    6. Olivier Delelis, Audrey Brussel, Pierre Sonigo
      Pages 155-170
    7. Alcina Nicol, Gerard J. Nuovo
      Pages 171-182
    8. Kenji Kashima, Tsutomu Daa, Shigeo Yokoyama
      Pages 183-189
    9. Feng Gao
      Pages 191-200
    10. Marie Gueudin, François Simon
      Pages 221-228

About this book

Introduction

Advances in our understanding of human retroviruses have been occurring at a very rapid pace and have contributed significantly to the development of new biotechnologies. In Human Retrovirus Protocols: Virology and Molecular Biology, recognized international researchers describe in detail the basic and state-of-the-art methods they have optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from proven methods for the isolation and detection of human retroviruses to advanced methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from peripheral blood mononuclear cells (PBMC), blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinant virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. The protocols follow the highly praised Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Human Retrovirus Protocols: Virology and Molecular Biology offers novice and experienced researchers alike proven tools essential for productive study and understanding of the human retroviruses.

Keywords

DNA Microarray PCR RNA Termination gene expression genes molecular biology reproducible techniques virology virus

Editors and affiliations

  • Tuofu Zhu
    • 1
  1. 1.Departments of Laboratory Medicine and MicrobiologyUniversity of Washington School of MedicineSeattle

Bibliographic information

  • DOI https://doi.org/10.1385/1592599079
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-495-1
  • Online ISBN 978-1-59259-907-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book