Chemosensitivity: Volume II

In VIVO Models, Imaging, and Molecular Regulators

  • Rosalyn D. Blumenthal

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 111)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Measurements of DNA Damage, Cell Death, and Regulators of Cytotoxicity

    1. Front Matter
      Pages 1-1
    2. Aslamuzzaman Kazi, Q. Ping Dou
      Pages 33-42
    3. Narendra P. Singh
      Pages 55-67
    4. Alok C. Bharti, Yasunari Takada, Bharat B. Aggarwal
      Pages 69-78
    5. Richard B. Lock, Kathleen M. Murphy
      Pages 83-96
    6. Pedro Mestres-Ventura, Andrea Morguet, Anette Schofer, Michael Laue, Werner Schmidt
      Pages 109-125
    7. Laura Gatti, Franco Zunino
      Pages 127-148
    8. Awtar Krishan, Ronald M. Hamelik
      Pages 149-166
  3. Genomics, Proteomics, and Chemosensitivity

    1. Front Matter
      Pages 195-195
    2. Julia Poland, Silke Wandschneider, Andrea Urbani, Sergio Bernardini, Giorgio Federici, Pranav Sinha
      Pages 267-281
  4. In VIVO Animal Modeling of Chemosensitivity

    1. Front Matter
      Pages 283-283
    2. Richard B. Lock, Natalia L. Liem, Rachael A. Papa
      Pages 323-334
    3. C. Patrick Reynolds, Bee-Chun Sun, Yves A. DeClerck, Rex A. Moats
      Pages 335-350
    4. Hayao Nakanishi, Seiji Ito, Yoshinari Mochizuki, Masae Tatematsu
      Pages 351-362
    5. Tarik Z. Belhocine, Francis G. Blankenberg
      Pages 363-380
    6. Richard Mazurchuk, Joseph A. Spernyak
      Pages 381-415
    7. Guy Jerusalem, Tarik Z. Belhocine
      Pages 417-440
  5. Back Matter
    Pages 441-442

About this book


Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe their best laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains today's best protocols for classifying tumors into response categories and for customizing therapy to individuals. These techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. Highlights include genomic and proteomic approaches to assess chemosensivity, in vivo imaging approaches to assess early response to therapy, and methods to statistically analyze data from in vivo therapy. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor.
Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more or less sensitive to a particular agent.


DNA apoptosis cell cell death chemoresistance genomics imaging magnetic resonance imaging (MRI) tumor

Editors and affiliations

  • Rosalyn D. Blumenthal
    • 1
  1. 1.Garden State Cancer CenterBellevilleUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-586-6
  • Online ISBN 978-1-59259-889-2
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site