Molecular Cardiology

Methods and Protocols

  • Zhongjie Sun

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 112)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages i-xv
  3. Gene Transfer and Gene Therapy for Cardiovascular Disease

    1. Front Matter
      Pages 13-13
    2. J. Mark Jones, Walter J.Koch
      Pages 15-35
    3. J. Mark Jones, Walter J. Koch
      Pages 49-57
    4. Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, Kaushik P. Patel
      Pages 59-79
    5. Åsa B. Gustafsson, Roberta A. Gottlieb, David J. Granville
      Pages 81-90
    6. Xiuqing Wang, Robert Cade, Zhongjie Sun
      Pages 91-107
    7. Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, Thomas J. Podor
      Pages 109-154
  4. Stem Cell Therapy for Cardiovascular Disease

    1. Front Matter
      Pages 173-173
    2. Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Mènard, Michel Puc
      Pages 175-182
    3. Sharon Etzion, Radka Holbova, Liron Miller, Jonathan Leor
      Pages 205-221
    4. Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus et al.
      Pages 223-238
    5. Hideki Iwaguro, Takayuki Asahara
      Pages 239-247
  5. Gene Analysis in the Injured and Hypertrophied Heart

    1. Front Matter
      Pages 249-249
    2. Arwen L. Hunter, Jonathan C. Choy, David J. Granville
      Pages 277-289
    3. Xiaoning Si, Maziar Rahmani, Ji Yuan, Honglin Luo
      Pages 291-303
    4. Bobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche et al.
      Pages 305-320
    5. Marcos E. Barbosa, Natalia Alenina, Michael Bader
      Pages 339-352
    6. Matthew J. Wolf, Hideo Tachibana, Howard A. Rockman
      Pages 353-362
  6. Transgenes in Cardiovascular Research

    1. Front Matter
      Pages 363-363
    2. Xia Shen, Gang Ye, Naira S. Metreveli, Paul N. Epstein
      Pages 379-388
  7. Back Matter
    Pages 405-419

About this book


The application of molecular technology to the heart opens a new and promising avenue for both the study and cure of heart diseases that were once thought to be incurable. In Molecular Cardiology: Methods and Protocols, leading cardiac researchers from around the world describe in detail their own proven, cutting-edge techniques for gene therapy and stem cell therapy for heart diseases. The authors provide readily reproducible methods for genetic dissection, signal transduction, and microarray analysis. The protocols include techniques for gene transfer to heart tissue using viral and nonviral vectors, the basis for gene therapy of heart diseases, and for producing transgenic models of heart diseases, the novel and powerful tools for the study of heart disease pathogenesis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells. The protocols offer step-by-step laboratory instructions, an introduction outlining the principle behind the technique, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Molecular Cardiology: Methods and Protocols offers basic and clinical researchers the first major collection of the state-of-the-art molecular and cell biology techniques for the study and treatment of genetic and acquired heart diseases.


cardiology cardiovascular gene therapy heart heart disease heart failure myocardial infarction vascular disease

Editors and affiliations

  • Zhongjie Sun
    • 1
  1. 1.Departments of Medicine and Physiology and Functional GenomicsCollege of Medicine, University of FloridaGainesville

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-363-3
  • Online ISBN 978-1-59259-879-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site