Amyloid Proteins

Methods and Protocols

  • Einar M. Sigurdsson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 299)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. In Vitro Assays

    1. Front Matter
      Pages 1-1
    2. Sabrina S. Vollers, David B. Teplow, Gal Bitan
      Pages 11-18
    3. Paula Saá, Joaquín Castilla, Claudio Soto
      Pages 53-65
    4. O. Sumner Makin, Louise C. Serpell
      Pages 67-80
    5. Aleksey Lomakin, David B. Teplow, George B. Benedek
      Pages 153-174
    6. Martin von Bergen, Li Li, Eckhard Mandelkow
      Pages 175-184
  3. Cell Culture Assays

    1. Front Matter
      Pages 195-195
    2. Finnbogi R. Thormodsson, Ingvar H. Olafsson
      Pages 197-210
    3. Frances Prelli, Monika Pawlik, Blas Frangione, Efrat Levy
      Pages 221-226
    4. Sylvain Lehmann
      Pages 227-234
  4. In Vivo-Related Assays

    1. Front Matter
      Pages 235-235

About this book

Introduction

In recent years, various in vitro and in vivo techniques, transgenic technologies, and novel animal models have been developed to understand amyloid diseases-such as Alzheimer's disease and some forms of diabetes-and to screen for drugs that can block amyloid formation and/or disassemble its associated fibrils. In Amyloid Proteins: Methods and Protocols, internationally known researchers describe in step-by-step detail their best techniques for studying these types of proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide proven methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Amyloid Proteins: Methods and Protocols offers both skilled and novice investigators a proven collection of standard and novel techniques for studying amyloid proteins and peptides, as well as their relation to amyloid diseases.

Keywords

ELISA Termination Vivo biology electron microscopy molecular biology proteins reproducible techniques

Editors and affiliations

  • Einar M. Sigurdsson
    • 1
  1. 1.Departments of Psychiatry and PathologyNew York University School of MedicineNew YorkNY

Bibliographic information

  • DOI https://doi.org/10.1385/1592598749
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-337-4
  • Online ISBN 978-1-59259-874-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book