Volume 1 In Vitro Assays

  • Rosalyn D. Blumenthal

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 110)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Rosalyn D. Blumenthal
    Pages 3-18
  3. Anupama Munshi, Marvette Hobbs, Raymond E. Meyn
    Pages 21-28
  4. Jack D. Burton
    Pages 69-78
  5. Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, Shiro Nozawa
    Pages 79-86
  6. Rita Grigoryan, Nino Keshelava, Clarke Anderson, C. Patrick Reynolds
    Pages 87-100
  7. Marylène Fortin, Ann-Muriel Steff, Patrice Hugo
    Pages 121-137
  8. Nino Keshelava, Tomás Frgala, Jirí Krejsa, Ondrej Kalous, C. Patrick Reynolds
    Pages 139-153
  9. Robert L. Ochs, Dennis Burholt, Paul Kornblith
    Pages 155-172
  10. John F.Gibbs, Youcef M.Rustum, Harry K.Slocum
    Pages 185-195
  11. Mitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, Seiji Kawasaki
    Pages 213-227
  12. Back Matter
    Pages 229-231

About this book


Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe state-of-the-art laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and nonadherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more, or less, sensitive to a particular agent.


cell cell culture cell death chemotherapy imaging tumor

Editors and affiliations

  • Rosalyn D. Blumenthal
    • 1
  1. 1.Garden State Cancer CenterBelleville

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-345-9
  • Online ISBN 978-1-59259-869-4
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site