Human Cell Culture Protocols

  • Joanna Picot

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 107)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, James A. Gallagher
    Pages 29-54
  3. Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, James A. Gallagher
    Pages 55-68
  4. Grace K. Pavlath, Emanuela Gussoni
    Pages 97-110
  5. Volker Meske, Frank Albert, Thomas G. Ohm
    Pages 111-123
  6. Vanessa van Harmelen, Thomas Skurk, Hans Hauner
    Pages 125-135
  7. Bice Perussia, Matthew J. Loza
    Pages 147-161
  8. Mark P. Mattson
    Pages 163-171
  9. Victor J. Turnbull
    Pages 173-182
  10. M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, Scott H. Randell
    Pages 183-206
  11. Carsten Ehrhardt, Kwang-Jin Kim, Claus-Michael Lehr
    Pages 207-216
  12. Pierre Chailler, Daniel Ménard
    Pages 217-236
  13. Martin Bayliss, Graham Somers
    Pages 249-267
  14. Heather M. Wilson, Keith N. Stewart
    Pages 269-282
  15. Per Hellman
    Pages 291-301
  16. Susan B. Curtis, Alison M. J. Buchan
    Pages 313-323
  17. Monica Berry, Marcus Radburn-Smith
    Pages 325-341
  18. Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman, Monte A. DelMonte, Douglas A. Greene
    Pages 343-351
  19. Back Matter
    Pages 353-359

About this book


In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.


genes phorbol ester regulation reproducible techniques tissue

Editors and affiliations

  • Joanna Picot
    • 1
  1. 1.SouthamptonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-222-3
  • Online ISBN 978-1-59259-861-8
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site