Methods and Protocols

  • Jérôme P. Fennell
  • Andrew H. Baker

Part of the Methods In Molecular Medicine™ book series (MIMM, volume 108)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Models Of Hypertension

    1. Front Matter
      Pages 1-1
    2. Delyth Graham, Martin W. McBride, Nicholas J. R. Brain, Anna F. Dominiczak
      Pages 3-15
    3. Michael Bader
      Pages 17-32
    4. Elena Popova, Michael Bader, Alexander Krivokharchenko
      Pages 33-50
    5. Klaas Kramer, René Remie
      Pages 51-62
  3. Assessment Of Free Radicals In Endothelial Dysfunction

    1. Front Matter
      Pages 63-63
    2. Michela Zanetti, Livius V. d’Uscio, Timothy E. Peterson, Zvonimir S. Katusic, Timothy O’Brien
      Pages 65-72
    3. Angela Spiers, Neal Padmanabhan
      Pages 91-104
  4. Nucleic Acid Techniques

    1. Front Matter
      Pages 105-105
    2. Carol Moreno, Kathleen Kennedy, Jaime Wendt Andrae, Howard J. Jacob
      Pages 131-138
    3. Ian Bird M.
      Pages 139-148
    4. Michihiro Yoshimura, Shota Nakamura, Hisao Ogawa
      Pages 189-198
    5. Klaus Lindpaintner
      Pages 235-260
  5. Proteins And Proteomics

    1. Front Matter
      Pages 261-261
    2. Soren Naaby-Hansen, Gayathri D., Claire Hastie, Piers Gallney, Rainer Cramer
      Pages 275-296
  6. Gene Transfer

    1. Front Matter
      Pages 297-297
    2. Sabyasachi Sen, Padraig M. Strappe, Timothy O’Brien
      Pages 299-314
    3. Marika Ruponen, Zanna Hyvönen, Arto Urtti, Seppo Ylä-Herttuala
      Pages 315-328
    4. M. Ian Phillips, Birgitta Kimura
      Pages 363-379
    5. Cezary Wójcik, Rosalind Fabunmi, George N. DeMartino
      Pages 381-393
    6. Lorraine M. Work, Campbell G. Nicol, Laura Denby, Andrew H. Baker
      Pages 395-413
  7. Stem Cells

    1. Front Matter
      Pages 415-415
    2. Kenneth R. Boheler, David G. Crider, Yelena Tarasova, Victor A. Maltsev
      Pages 417-435
  8. Bioinformatics

    1. Front Matter
      Pages 437-437
    2. Stephen A. Krawetz, Sorin Draghici, Robert Goodrich, Zhandong Liu, G. Charles Ostermeier
      Pages 439-458
  9. Back Matter
    Pages 487-501

About this book


Our understanding of the pathogenesis of hypertension and the development of new therapies for its management have each proven to be directly dependent on the availability and application of novel molecular biological methodologies. In Hypertension: Methods and Protocols, a panel of recognized experts from key institutions around the world describes in step-by-step detail many new and essential molecular techniques for cardiovascular hypertension research. The methods presented range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using adenoviral and nonviral (polymers, liposomes, and antisense agents) vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. A review of bioinformatic resources for pharmacogenomics describes the application of in silico strategies to identify nuclear matrix attachment regions. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Hypertension: Methods and Protocols offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.


blood pressure cardiovascular gene therapy genetics heart hypertension vascular disease

Editors and affiliations

  • Jérôme P. Fennell
    • 1
  • Andrew H. Baker
    • 2
  1. 1.Moyne Institute for Preventive MedicineTrinity CollegeDublinIreland
  2. 2.Division of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research CentreUniversity of GlasgowGlasgowUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-323-7
  • Online ISBN 978-1-59259-850-2
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site