Microbial Processes and Products

  • José-Luis Barredo

Part of the Methods in Biotechnology book series (MIBT, volume 18)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Guillaume E. Vanot, Michelle Sergent
    Pages 25-39
  3. José M. Luengo, Belén GarcÍa, Ángel Sandoval, Elsa Arias-Barrau, Sagrario Arias, Francisco Bermejo et al.
    Pages 91-129
  4. Maria Margarida Diogo, João Ant′onio Queiroz, Duarte Miguel F. Prazeres
    Pages 165-178
  5. Masato Ikeda, Junko Ohnishi, Satoshi Mitsuhashi
    Pages 179-190
  6. Heiko Barg, Marco Malten, Martina Jahn, Dieter Jahn
    Pages 205-223
  7. Heidi Sisniega, José-Luis del Río, María-José Amaya, Ignacio Faus
    Pages 225-237
  8. Enrique A., Tamás Papp, Jesper Breum, José Arnau, Arturo P.
    Pages 239-256
  9. Hans Visser, Gerhard Sandmann, Jan C. Verdoes
    Pages 257-272
  10. Paul D. Fraser, Peter M. Bramley
    Pages 273-282
  11. MarÍa A. Santos, Laura Mateos, Karl-Peter Stahmann, José-Luis Revuelta
    Pages 283-300
  12. Stefania Cresti, Cesira L. Galeotti, Serena Schippa, Gian Maria Rossolini, Maria C. Thaller
    Pages 313-331
  13. Bart Weimer, Yi Xie, Lan-szu Chou, Adele Cutler
    Pages 333-343
  14. Tammi L. Olineka, Apostolos Spiropoulos, Paula A. Mara, Linda F. Bisson
    Pages 345-368
  15. Frédéric Leroy, Luc De Vuyst
    Pages 369-380
  16. Eduardo J. Schiffrin, Nabila Ibnou-Zekri, Jean M. Ovigne, Thierry von der Weid, Stephanie Blum
    Pages 381-395
  17. Bart Weimer, Ben Dias
    Pages 397-403
  18. Axel Schippers, Klaus Bosecker
    Pages 405-412
  19. Marion Paterson-Beedle, Lynne E. Macaskie
    Pages 413-436
  20. Vera Weihs, Christine Rohde
    Pages 437-450
  21. Back Matter
    Pages 495-515

About this book


The development of recombinant DNA techniques over the last 20 years has greatly expanded the opportunities for using microorganisms to produce a broad range of valuable substances. In Microbial Processes and Products, outstanding leaders in using microorganisms as cell factories describe in detail their best laboratory procedures for many processes and products mediated by microorganisms. An overview chapter describes how to develop strain improvement programs and strategies to optimize fermentation processes. Taking advantage of the most recent developments in such processes, the authors offer step-by-step experimental methods for the optimal design of microbial metabolite production, including semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination, L-lysine, vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids b-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation. Additionally, the use of phenylacetyl-CoA catabolon for enzymatic synthesis of penicillins, aromatic biotransformations, synthesis of new bioplastics, biosensor design, or synthesis of drug vehicles, and the development of a phosphate encoding gene as a reporter and to monitor gene expression are illustrated.
The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection. Additional chapters offer techniques for analysis of antimicrobial metabolites and carotenoids, volatile sulfur compounds, metabolic pathway fluxes, gene expression arrays, proteome analysis, bacterial modulation of the innate immune response, bioleaching activity, and heavy metal remediation. Finally, three overview chapters on transport of biological material, deposit of biological material for patent purposes, and protection of biotechnological inventions are shown. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Enzymes and Biotransformations, describes in detail cutting-edge techniques for the screening, evolution, production, immobilization, and use of enzymes.
Wide-ranging and practical, Microbial Processes and Products offers laboratory and industrial scientists a wealth of readily reproducible techniques for the successful microbial generation of biochemical products to serve the needs of human health, nutrition, and environmental protection.


Acetyl-CoA Bioleaching Biosensor Expression Fermentation Lysin Polysaccharide biotechnology gene expression gene therapy microbiology microorganism mutagenesis protein transport

Editors and affiliations

  • José-Luis Barredo
    • 1
  1. 1.R&D Biology Antibióticos S. A.LeónSpain

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-548-4
  • Online ISBN 978-1-59259-847-2
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site