Microbial Enzymes and Biotransformations

  • José Luis Barredo

Part of the Methods in Biotechnology book series (MIBT, volume 17)

Table of contents

  1. Front Matter
    Pages i-xi
  2. José L. Adrio, Arnold L. Demain
    Pages 1-27
  3. Steven J. Setford, Jeffrey D. Newman
    Pages 29-60
  4. Susanne Brakmann, Sascha Nico Stumpp
    Pages 61-74
  5. Abdulhameed Sabu, K. Madhavan Nampoothiri, Ashok Pandey
    Pages 75-90
  6. Hemraj S. Nandanwar, Gurinder S. Hoondal, Rakesh M. Vohra
    Pages 91-104
  7. Oskar Bañuelos, Maria del Carmen Ronchel, José Luis Adrio, Javier Velasco
    Pages 105-113
  8. José A. Gil, Angelina Ramos, Sirin A. I. Adham, Noelia Valbuena, Michal Letek, Luis M. Mateos
    Pages 115-139
  9. Susumu Ito, Tohru Kobayashi, Yuji Hatada, Koki Horikoshi
    Pages 151-161
  10. Chandran Sandhya, K. Madhavan Nampoothiri, Ashok Pandey
    Pages 165-179
  11. Encarnación Mellado, Cristina Sánchez-Porro, Antonio Ventosa
    Pages 181-190
  12. Rupinder Tewari, Ram P. Tewari, Gurinder S. Hoondal
    Pages 191-208
  13. Andrea Weckbecker, Werner Hummel
    Pages 225-238
  14. Scott J. Novick, J. David Rozzell
    Pages 247-271
  15. Cesar Mateo, Valeria Grazu, Olga Abian, Manuel Fuentes, Gloria Fernández-Lorente, José M. Palomo et al.
    Pages 273-288
  16. Thomas M. S. Chang
    Pages 289-306
  17. Back Matter
    Pages 307-319

About this book


The application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms.
Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.


Archaea Biosensor Expression Glucose Oligosaccharide Phenylalanin biocatalysis microorganism polymer preparation

Editors and affiliations

  • José Luis Barredo
    • 1
  1. 1.R & D BiologyAntibióticos S. ALeón

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-253-7
  • Online ISBN 978-1-59259-846-5
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site